From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07500 [old locus tag: NWMN_1332 ]
  • pan locus tag?: SAUPAN003849000
  • symbol: NWMN_RS07500
  • pan gene symbol?: ctpA
  • synonym:
  • product: serine protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07500 [old locus tag: NWMN_1332 ]
  • symbol: NWMN_RS07500
  • product: serine protease
  • replicon: chromosome
  • strand: -
  • coordinates: 1465657..1467147
  • length: 1491
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1465657..1467147) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1332

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATGATAAGCAACACACATCTTCATCCGATGATGAACGCGCTGAAATTGCAACAAGC
    AATCAAGACCAAGAAACTAATTCATCGAAACGCGTTCACTTAAAACGTTGGCAATTCATA
    TCAATATTAATTGGTACAATCCTAATAACAGCTGTCATCACAGTTGTTGCATATATTTTT
    ATAAATCAAAAAATAAGTGGTTTAAACAAAACTGATCAAGCAAACTTAAATAAAATTGAA
    AATGTGTATAAAATCTTAAATAGTGATTATTACAAAAAACAGGACTCTGACAAGTTAAGT
    AAAGCTGCAATTGATGGCATGGTCAAAGAATTAAAAGATCCTTATTCTGAATATTTAACA
    AAAGAACAAACGAAATCCTTTAATGAAGGTGTTTCAGGTGATTTTGTAGGTATTGGTGCA
    GAAATGCAAAAGAAAAATGATCAAATTATGGTTACTAGTCCTATGAAGGGATCTCCAGCA
    GAACGTGCTGGCATTCGTCCTAAAGATGTCATTACTAAAGTAAATGGAAAATCAATTAAA
    GGTAAAGCATTAGATGAAGTTGTCAAAGATGTTCGTGGTAAAGAAAACACTGAAGTCACT
    TTAACTGTTCAACGAGGTAGTGAAGAAAAAGACGTTAAGATTAAACGTGAAAAAATTCAT
    GTTAAAAGTGTTGAGTATAAGAAAAAAGGTAAAGTTGGAGTTATTACTATTAATAAATTC
    CAGAATGATACATCAGGTGAATTGAAAGATGCAGTTCTAAAAGCTCACAAAGATGGTTTG
    AAAAAGATTGTTTTAGATTTAAGAAATAATCCAGGTGGACTACTAGATGAAGCTGTTAAA
    ATGGCAAATATTTTTATCGATAAAGGAAAAACTGTTGTTAAACTAGAAAAAGGTAAAGAT
    ACTGAAGCAATTCAAACTTCTAATGATGCGTTAAAAGAAGCGAAAGACATGGATATATCC
    ATCTTAGTGAATGAAGGTTCAGCTAGCGCTTCTGAAGTGTTTACTGGTGCGCTAAAAGAC
    TATAATAAAGCTAAAGTTTATGGGTCAAAAACATTCGGCAAAGGTGTCGTACAAACTACA
    AGAGAGTTTAAGGATGGTTCATTGTTAAAATATACTGAAATGAAATGGTTAACGCCAGAT
    GGTCATTATATTCACGGTAAAGGCATTAAACCAGACGTTACTATTGACACACCTAAATAT
    CAATCTTTAAATGTCATTCCTAATACGAAAACATTTAAAGTTGGAGACGATGATAAAAAT
    ATTAAAACTATTAAAATTGGTTTATCAGCTTTAGGTTATAAAGTTGATAATGAATCAACG
    CAATTTGATAAAGCTTTAGAAAATCAAGTTAAAGCTTTCCAACAAGCGAATAAACTTGAG
    GTAACTGGTGAATTTAATAAAGAAACGAATAATAAATTTACTGAGTTATTAGTTGAAAAA
    GCTAATAAACATGATGATGTTCTCGATAAGTTGATTAATATTTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1491

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS07500 [old locus tag: NWMN_1332 ]
  • symbol: NWMN_RS07500
  • description: serine protease
  • length: 496
  • theoretical pI: 9.7491
  • theoretical MW: 55260.6
  • GRAVY: -0.634476

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 344)
    Genetic information processing Protein fate Protein modification and repair C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 344)
    and 8 more
    putative carboxyl-terminal-processing protease, deltaproteobacterial (TIGR03900; EC 3.4.21.-; HMM-score: 264.4)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 36.4)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 36.4)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 28.8)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 20.2)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 20.2)
    Cellular processes Cellular processes Sporulation and germination spore cortex-lytic enzyme (TIGR02869; HMM-score: 18.7)
    Signal transduction Regulatory functions Protein interactions anti-anti-sigma factor (TIGR00377; HMM-score: 12.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ClpP_crotonase (CL0127) Peptidase_S41; Peptidase family S41 (PF03572; HMM-score: 165.5)
    and 7 more
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 56.2)
    PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 51.7)
    PGBD (CL0244) PG_binding_1; Putative peptidoglycan binding domain (PF01471; HMM-score: 46.5)
    PDZ-like (CL0466) Tricorn_PDZ; Tricorn protease PDZ domain (PF14685; HMM-score: 17)
    PGBD (CL0244) PG_binding_2; Putative peptidoglycan binding domain (PF08823; HMM-score: 14.6)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 12.7)
    no clan defined DUF3394; Domain of unknown function (DUF3394) (PF11874; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002498
    • TAT(Tat/SPI): 0.000562
    • LIPO(Sec/SPII): 0.00446
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILIGTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKAAIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPAERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREKIHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLDEAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVFTGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPDVTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQVKAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=NWMN_RS07500&oldid=77262"