From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS07745 [old locus tag: NWMN_1376 ]
  • pan locus tag?: SAUPAN003925000
  • symbol: NWMN_RS07745
  • pan gene symbol?: aroB
  • synonym:
  • product: 3-dehydroquinate synthase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS07745 [old locus tag: NWMN_1376 ]
  • symbol: NWMN_RS07745
  • product: 3-dehydroquinate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1542165..1543229
  • length: 1065
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (1542165..1543229) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1376

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAATTACAAACAACATACCCTTCAAATAATTATCCAATATATGTTGAACACGGTGCA
    ATTGACCATATTAGCACGTATATTGATCAGTTTGATCAAAGTTTTATATTAATTGACGAG
    CATGTAAATCAATATTTTGCTGATAAATTTGATGATATTTTATCATATGAAAATGTACAT
    AAAGTTATTATTCCAGCTGGTGAAAAGACGAAAACATTTGAGCAATATCAAGAAACATTA
    GAGTATATTTTATCCCATCATGTAACTCGTAATACAGCAATTATTGCTGTTGGTGGTGGT
    GCAACTGGTGATTTTGCTGGGTTTATTGCGGCGACACTTTTACGAGGCGTTCACTTTATA
    CAAGTGCCAACGACTATACTAGCGCATGATTCTAGTGTTGGCGGTAAAGTGGGTATTAAC
    TCAAAGCAAGGTAAAAACCTTATCGGTGCATTTTATCGTCCAACTGCTGTGATTTATGAT
    TTAGACTTTTTAAAGACGTTACCATTTGAGCAAATATTAAGTGGCTATGCAGAAGTTTAT
    AAGCATGCGTTATTGAATGGTGAATCAGCGACGCAAGATATCGAACAGCACTTTAAAGAT
    AGAGAGATATTACAGTCATTAAATGGTATGGATAAATATATTGCTAAAGGTATTGAAACG
    AAGCTGGATATTGTTATTGCAGATGAAAAAGAACAAGGTGTACGTAAATTTTTAAATTTA
    GGTCATACATTTGGTCATGCTGTTGAATACTATCATAAAATACCTCATGGTCATGCAGTG
    ATGGTTGGCATTATCTATCAATTTATAGTTGCGAATGCTTTGTTTGATTCTAAGCATGAT
    ATTAATCATTATATTCAATATTTAATACAACTCGGCTATCCTTTAGACATGATAACTGAC
    TTGGATTTTGAAACGTTATACCAATATATGCTAAGTGATAAAAAGAATGATAAGCAAGGT
    GTACAAATGGTCTTGATTAGACAATTTGGAGATATCGTTGTACAACATGTTGATCAACTA
    ACATTACAACATGCATGTGAACAATTAAAAACATATTTTAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1065

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS07745 [old locus tag: NWMN_1376 ]
  • symbol: NWMN_RS07745
  • description: 3-dehydroquinate synthase
  • length: 354
  • theoretical pI: 6.09151
  • theoretical MW: 40315.6
  • GRAVY: -0.156497

Function[edit | edit source]

  • reaction:
    EC 4.2.3.4?  ExPASy
    3-dehydroquinate synthase 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-dehydroquinate synthase (TIGR01357; EC 4.2.3.4; HMM-score: 376)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Lipoate putative sugar phosphate phospholyase (cyclizing) (TIGR04425; EC 4.2.3.-; HMM-score: 235.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    DHQS (CL0224) DHQ_synthase; 3-dehydroquinate synthase (PF01761; HMM-score: 289.9)
    and 2 more
    Fe-ADH_2; Iron-containing alcohol dehydrogenase (PF13685; HMM-score: 59.4)
    Fe-ADH; Iron-containing alcohol dehydrogenase (PF00465; HMM-score: 52.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Co2+, NAD+, Zn2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00262
    • TAT(Tat/SPI): 0.000078
    • LIPO(Sec/SPII): 0.000281
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQYFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGATGDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYDLDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGIETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFDSKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVVQHVDQLTLQHACEQLKTYFK

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]