From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08110 [old locus tag: NWMN_1437 ]
  • pan locus tag?: SAUPAN004110000
  • symbol: NWMN_RS08110
  • pan gene symbol?: lipM
  • synonym:
  • product: octanoyltransferase LipM

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS08110 [old locus tag: NWMN_1437 ]
  • symbol: NWMN_RS08110
  • product: octanoyltransferase LipM
  • replicon: chromosome
  • strand: -
  • coordinates: 1607046..1607876
  • length: 831
  • essential: unknown

Accession numbers[edit | edit source]

  • Location: NC_009641 (1607046..1607876) NCBI
  • BioCyc:
  • MicrobesOnline:

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACTGAAACTTGGAATTTTATTAATACAGGAAGTAAAGACCCATATTATAATATGGCA
    ATGGATGAAGCGTTATTAAATTTTGTCTCTAGAGGTGAAATTGATCCAGTTATACGTTTT
    TACACATGGAATCCTGCAACATTATCAATAGGGTATTTCCAAAGGCTACAAAAAGAAATT
    GATATTGATAAGGTTAAAGAGAAAGGTTTTGGTCTCGTAAGGCGTCAAACTGGTGGCCGT
    GGGGTGCTACATGATAAAGAGTTAACTTACAGTGTGATTGTGCCTGAATCGCATCCGAAT
    ATGCCTTCTACAGTTACAGAAGCGTACAGAGTGATTTCACAAGGATTATTAGAAGGATTT
    AAAAACTTAGGATTTGATACTTATTTTGCGGTACCTAAAACGCCTGAAGAGCGTCAAAAA
    TTAAAACAACCTCGAAGTTCAGTGTGTTTTGATGCACCAAGTTGGTATGAGCTAGTTGTT
    GAAGGCCGTAAGATTGCTGGGAGTGCTCAAACGAGACAAAAGGGCGTAATCCTCCAACAC
    GGTTCGATTTTACAAGATATTGATATTGATGAACTGTTTGATATGTTTATTTATAAAAAT
    GAAAGATTAAAATTAAAAATGAAAGAGGCATTCGTTGAAAAAGCAGTTGCCATCAATGAT
    ATTTCAGATGAACACATTACTATTAGTCAAATGGAAGAAGCTTTCGAGAAAGGTTTTAAG
    AAAGGTTTAAATATAGAATTAAAGCCACTTGAATTAACAGAAGCTCAATTGGCAGAAGTG
    GAAGAATTAACTGAAAAATATCGTTCAGATGAATGGATGTTTAGAAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    831

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_RS08110 [old locus tag: NWMN_1437 ]
  • symbol: NWMN_RS08110
  • description: octanoyltransferase LipM
  • length: 276
  • theoretical pI: 5.15375
  • theoretical MW: 31881.2
  • GRAVY: -0.473913

Function[edit | edit source]

  • reaction:
    EC 2.3.1.181?  ExPASy
    Lipoyl(octanoyl) transferase Octanoyl-[acyl-carrier-protein] + protein = protein N6-(octanoyl)lysine + [acyl-carrier-protein]
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 101)
  • TheSEED:
  • PFAM:
    tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 95.4)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002278
    • TAT(Tat/SPI): 0.000151
    • LIPO(Sec/SPII): 0.000409
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

  • GI: 446063254 NCBI
  • RefSeq: WP_000141109 NCBI
  • UniProt:

Protein sequence[edit | edit source]

  • MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWNPATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMPSTVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVVEGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAINDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]