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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS08110 [old locus tag: NWMN_1437 ]
- pan locus tag?: SAUPAN004110000
- symbol: NWMN_RS08110
- pan gene symbol?: lipM
- synonym:
- product: octanoyltransferase LipM
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS08110 [old locus tag: NWMN_1437 ]
- symbol: NWMN_RS08110
- product: octanoyltransferase LipM
- replicon: chromosome
- strand: -
- coordinates: 1607046..1607876
- length: 831
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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781ATGACTGAAACTTGGAATTTTATTAATACAGGAAGTAAAGACCCATATTATAATATGGCA
ATGGATGAAGCGTTATTAAATTTTGTCTCTAGAGGTGAAATTGATCCAGTTATACGTTTT
TACACATGGAATCCTGCAACATTATCAATAGGGTATTTCCAAAGGCTACAAAAAGAAATT
GATATTGATAAGGTTAAAGAGAAAGGTTTTGGTCTCGTAAGGCGTCAAACTGGTGGCCGT
GGGGTGCTACATGATAAAGAGTTAACTTACAGTGTGATTGTGCCTGAATCGCATCCGAAT
ATGCCTTCTACAGTTACAGAAGCGTACAGAGTGATTTCACAAGGATTATTAGAAGGATTT
AAAAACTTAGGATTTGATACTTATTTTGCGGTACCTAAAACGCCTGAAGAGCGTCAAAAA
TTAAAACAACCTCGAAGTTCAGTGTGTTTTGATGCACCAAGTTGGTATGAGCTAGTTGTT
GAAGGCCGTAAGATTGCTGGGAGTGCTCAAACGAGACAAAAGGGCGTAATCCTCCAACAC
GGTTCGATTTTACAAGATATTGATATTGATGAACTGTTTGATATGTTTATTTATAAAAAT
GAAAGATTAAAATTAAAAATGAAAGAGGCATTCGTTGAAAAAGCAGTTGCCATCAATGAT
ATTTCAGATGAACACATTACTATTAGTCAAATGGAAGAAGCTTTCGAGAAAGGTTTTAAG
AAAGGTTTAAATATAGAATTAAAGCCACTTGAATTAACAGAAGCTCAATTGGCAGAAGTG
GAAGAATTAACTGAAAAATATCGTTCAGATGAATGGATGTTTAGAAAGTAG60
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831
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS08110 [old locus tag: NWMN_1437 ]
- symbol: NWMN_RS08110
- description: octanoyltransferase LipM
- length: 276
- theoretical pI: 5.15375
- theoretical MW: 31881.2
- GRAVY: -0.473913
⊟Function[edit | edit source]
- reaction: EC 2.3.1.181? ExPASyLipoyl(octanoyl) transferase Octanoyl-[acyl-carrier-protein] + protein = protein N6-(octanoyl)lysine + [acyl-carrier-protein]
- TIGRFAM: Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 101)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 95.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002278
- TAT(Tat/SPI): 0.000151
- LIPO(Sec/SPII): 0.000409
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWNPATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMPSTVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVVEGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAINDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY see NWMN_1437
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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