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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS08585 [old locus tag: NWMN_1529 ]
- pan locus tag?: SAUPAN004230000
- symbol: NWMN_RS08585
- pan gene symbol?: rarA
- synonym:
- product: recombinase RarA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS08585 [old locus tag: NWMN_1529 ]
- symbol: NWMN_RS08585
- product: recombinase RarA
- replicon: chromosome
- strand: +
- coordinates: 1693438..1694712
- length: 1275
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261GTGAGTACAGAACCATTAGCATCGAGAATGCGCCCAAAAAATATAGATGAAATCATTTCC
CAACAACATTTAGTTGGACCAAGAGGCATTATCAGAAGAATGGTTGATACAAAAAAATTA
ACTTCAATGATTTTTTATGGTCCACCTGGTATAGGCAAAACAAGTATTGCCAAAGCAATT
TCGGGCAGTACGCAATATAAATTCAGACAATTGAATGCTGTAACTAACACTAAAAAAGAT
ATGCAACTTGTTGTTGAAGAAGCTAAAATGTCTGGTCAAGTTATCTTGTTATTAGATGAA
ATACATCGACTAGATAAAGCTAAACAAGACTTTTTATTACCTCATTTAGAAAATGGCAAA
ATCGTCTTGATCGGTGCTACAACTTCAAATCCTTATCATGCTATCAATCCAGCGATTCGT
TCAAGAGCGCAAATTTTCGAGTTATATCCTTTAAATGACGAAGATGTGCGCCAAGCGTTA
ACTCGTGCAATAGAAGATGATGAGAATGGTTTGAAAACATATCAACCCAAAATTGATGAA
GATGCCATGACCTACTTTTCTACACAAAGCCAAGGTGATGTTCGTAGTGCGTTAAATGCA
TTGGAATTAGCTGTATTAAGCGCAGATAATGACAAAGACGGTTATCGACATGTTACATTG
CAAGATGCTAAAGACTGTTTACAAAAAGGTGCATTTGTAAGTGACAAGGATGGTGACATG
CATTACGATGTTATGAGCGCTTTCCAAAAATCTATCCGTGGTAGCGACGTCAATGCCGCT
TTACATTATTTAGCACGATTAATTGAAGCTGGAGATTTACCTACAATAGTTCGACGATTA
CTTGTAATTAGCTATGAGGATATAGGCTTAGCCTCACCTAATGCTGGTCAGAGAACACTT
GCTGCTATTGAATCAGCAGAACGTCTAGGTTTACCAGAAGCTAGAATTCCACTAAGCCAA
GCAGTAATCGAACTATGCTTATCACCTAAGTCAAATTCAGCAATGAGTGCCATTGATAGT
GCATTGTCCGATATTAGAAACGGTCATGTGGGCCAAATTCCAAACCATTTAAAAGATGGA
CATTATCAAGGTGCTAAAGATCTAGGCCGATCTATTGGTTACAAATATCCACACCAATAT
GTTAATGGCTATGTTTCACAGCAATATTTACCTGATAAACTAAAAAACAAAATTTATTAC
GAACCAAAAACGACATCTAAAAGTGAACAACAACTCAAAGAAATATATAACAACTTACTT
AAACAAAGGCCGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS08585 [old locus tag: NWMN_1529 ]
- symbol: NWMN_RS08585
- description: recombinase RarA
- length: 424
- theoretical pI: 8.51483
- theoretical MW: 47250.5
- GRAVY: -0.452123
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 68.7)and 26 moreCellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 47.7)Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 47.7)DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 45.1)Cellular processes Sporulation and germination ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 34.7)Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 34.7)DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 34)DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 30.4)DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 25.6)Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent Clp protease ATP-binding subunit ClpA (TIGR02639; HMM-score: 25.1)Protein fate Degradation of proteins, peptides, and glycopeptides endopeptidase La (TIGR00763; EC 3.4.21.53; HMM-score: 23.6)Protein fate Protein folding and stabilization ATP-dependent chaperone protein ClpB (TIGR03346; HMM-score: 20.8)AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 19.9)Cellular processes Cell division ATP-dependent metallopeptidase HflB (TIGR01241; EC 3.4.24.-; HMM-score: 18.7)Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent metallopeptidase HflB (TIGR01241; EC 3.4.24.-; HMM-score: 18.7)26S proteasome subunit P45 family (TIGR01242; HMM-score: 18.5)Protein fate Degradation of proteins, peptides, and glycopeptides proteasome ATPase (TIGR03689; EC 3.6.4.8; HMM-score: 18)putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 16.5)Protein fate Protein and peptide secretion and trafficking type VII secretion AAA-ATPase EccA (TIGR03922; HMM-score: 16)Mobile and extrachromosomal element functions Prophage functions phage nucleotide-binding protein (TIGR01618; HMM-score: 14.9)Cellular processes Sporulation and germination stage V sporulation protein K (TIGR02881; HMM-score: 14.9)Cellular processes Other gas vesicle protein GvpN (TIGR02640; HMM-score: 14.8)DNA metabolism DNA replication, recombination, and repair checkpoint protein rad24 (TIGR00602; HMM-score: 13.9)DNA metabolism DNA replication, recombination, and repair DNA replication and repair protein RecF (TIGR00611; HMM-score: 13.9)Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions adenylate kinase (TIGR01351; EC 2.7.4.-; HMM-score: 12.9)Protein fate Degradation of proteins, peptides, and glycopeptides putative ATP-dependent protease (TIGR00764; HMM-score: 12.4)Unknown function General Mg chelatase-like protein (TIGR00368; HMM-score: 11.7)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: post-AAA (CL0604) MgsA_C; MgsA AAA+ ATPase C terminal (PF12002; HMM-score: 216.6)and 26 moreno clan defined AAA_assoc_2; AAA C-terminal domain (PF16193; HMM-score: 85.6)P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 61.4)RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 51.4)AAA_14; AAA domain (PF13173; HMM-score: 28.6)Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 28.4)AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 25.6)AAA_16; AAA ATPase domain (PF13191; HMM-score: 24.5)Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 20.8)DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 20.6)TIP49; TIP49 C-terminus (PF06068; HMM-score: 19.8)AAA_22; AAA domain (PF13401; HMM-score: 19.6)ABC_tran; ABC transporter (PF00005; HMM-score: 19.3)Sigma54_activ_2; Sigma-54 interaction domain (PF14532; HMM-score: 19.3)Rad17; Rad17 cell cycle checkpoint protein (PF03215; HMM-score: 17.8)AAA_18; AAA domain (PF13238; HMM-score: 16.7)AAA_19; AAA domain (PF13245; HMM-score: 16.6)IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 15.8)AAA_3; ATPase family associated with various cellular activities (AAA) (PF07726; HMM-score: 15.5)RNA_helicase; RNA helicase (PF00910; HMM-score: 14.1)AAA_24; AAA domain (PF13479; HMM-score: 14.1)no clan defined LXG; LXG domain of WXG superfamily (PF04740; HMM-score: 14)P-loop_NTPase (CL0023) Parvo_NS1; Parvovirus non-structural protein NS1 (PF01057; HMM-score: 13.8)NB-ARC; NB-ARC domain (PF00931; HMM-score: 13.1)ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 13)AAA_2; AAA domain (Cdc48 subfamily) (PF07724; HMM-score: 12.7)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010866
- TAT(Tat/SPI): 0.004874
- LIPO(Sec/SPII): 0.000653
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMIFYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIHRLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQALTRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHVTLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTIVRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSAMSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPDKLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
NWMN_RS02025 single-stranded DNA-binding protein [1] (data from MRSA252) NWMN_RS02915 50S ribosomal protein L1 [1] (data from MRSA252) NWMN_RS02960 elongation factor G [1] (data from MRSA252) NWMN_RS02965 elongation factor Tu [1] (data from MRSA252) NWMN_RS04590 NADH dehydrogenase [1] (data from MRSA252) NWMN_RS05380 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) NWMN_RS05385 pyruvate dehydrogenase E1 component subunit beta [1] (data from MRSA252) NWMN_RS05390 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex [1] (data from MRSA252) NWMN_RS05395 dihydrolipoyl dehydrogenase [1] (data from MRSA252) NWMN_RS06490 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_RS07455 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [1] (data from MRSA252) NWMN_RS07460 2-oxoglutarate dehydrogenase E1 component [1] (data from MRSA252) NWMN_RS07605 serine/threonine dehydratase [1] (data from MRSA252) NWMN_RS08350 molecular chaperone DnaK [1] (data from MRSA252) NWMN_RS08685 50S ribosomal protein L21 [1] (data from MRSA252) NWMN_RS08930 pyruvate kinase [1] (data from MRSA252) NWMN_RS08995 universal stress protein UspA [1] (data from MRSA252) NWMN_RS11720 fructose-bisphosphate aldolase [1] (data from MRSA252) NWMN_RS12330 50S ribosomal protein L15 [1] (data from MRSA252) NWMN_RS12340 30S ribosomal protein S5 [1] (data from MRSA252) NWMN_RS12425 50S ribosomal protein L3 [1] (data from MRSA252) NWMN_RS14560 arginine deiminase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)