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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_RS09345 [old locus tag: NWMN_1665 ]
- pan locus tag?: SAUPAN004464000
- symbol: NWMN_RS09345
- pan gene symbol?: arsB
- synonym:
- product: arsenical efflux pump membrane protein ArsB
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_RS09345 [old locus tag: NWMN_1665 ]
- symbol: NWMN_RS09345
- product: arsenical efflux pump membrane protein ArsB
- replicon: chromosome
- strand: +
- coordinates: 1861621..1862913
- length: 1293
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261ATGATGACAACTTTAGCGACACTCATTTTTCTAGTAACTTTATTATTTGTATTATGGCAA
CCTAAAGGCCTAGATATTGGCATTACCGCACTAACTGGTGCCTTTATTGCTGTTATTACT
GGTGTTGTAAGTTTTTCCGATGTTTTCGAAGTAACAGGTATTGTTTGGAATGCTACTTTG
ACTTTTGTCTCAGTCATTCTTATTTCATTAATATTAGATAAAGTTGGATTATTCGAATGG
TCAGCTATTCACATGCTTCATGCTTCAAAAGGCAATGGTTTAAAAATGTTCGTTTATATC
ATATTATTGGGTGCCATTGTTGCTGCATTTTTCGCAAATGATGGCGCAGCGTTAATCTTA
ACGCCTATTGTATTAGCGATGGTTAAAAATATAGGTTTTAGTAAGCGGGCCATATTCCCT
TTTATTATTGCGAGTGGTTTTATAGCTGACACAACTTCTTTACCTTTAATCGTGAGCAAT
CTAGTGAATATTATATCTGCTGATTATTTTCATGTAGGATTCGTTCGATATTTTAGTAGA
ATGATTATACCTAATTTATTCTCGCTTTTAGCAAGTATTATAGTATTGTGGTTATATTTT
AGAAAAGCGATACCTAAAACGTTTGATGATAATAATATAAAGCATCCTAAAGATGCCATT
AATGATTTAAAGCTATTTAAAATTTCATGGATTGTTCTAGTTATATTACTTTTCGGCTAT
CTAATCAGTGAATTTACTAAAATTCCGGTATCAATTTTCACTGGAATCATTGCTTTTATT
TTTCTAATGTTGGCTCGTAAATCAAATGCTGTAAATATTAAGCAAGTCATTAAGGGCGCA
CCTTGGAATATAGTATTATTTTCAATTGGTATGTATATCGTCGTATTCGGCTTAAGAAAT
GCTGGCATTACTTTAATATTGGCTAAAATATTAGAATATATTTCCAATTACGGTCTATTT
AGCACTATTTTGGGAATGGGCTTCATTTCAGCGTTTTTATCATCAATAATGAATAATATG
CCTACAGTTTTAATAGATGCGATTGCTATTGGTCAATCAAATGTCCATGGCATGTTAAAA
GAAGGCCTAATTTATGCGAATGTTATCGGTTCTGATTTAGGTCCAAAAATTACACCGATA
GGCTCTTTAGCTACATTACTGTGGTTACACGTCTTAACACAAAAAGATGTTAAGATTTCT
TGGGGCACATACTTTAAAACTGGTATCATCATTACAATTCCAGTACTATTTATAACCCTC
ATAGGGTTGTATCTAACACTTATCATATTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_RS09345 [old locus tag: NWMN_1665 ]
- symbol: NWMN_RS09345
- description: arsenical efflux pump membrane protein ArsB
- length: 430
- theoretical pI: 10.0887
- theoretical MW: 47271.7
- GRAVY: 1.09023
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Detoxification arsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 706.2)Transport and binding proteins Other arsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 706.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: IT (CL0182) ArsB; Arsenical pump membrane protein (PF02040; HMM-score: 629.2)and 1 moreCitMHS; Citrate transporter (PF03600; HMM-score: 90.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.045146
- TAT(Tat/SPI): 0.000324
- LIPO(Sec/SPII): 0.012363
- predicted transmembrane helices (TMHMM): 11
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVSFSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIILLGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSNLVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKDAINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQVIKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLSSIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVLTQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: ArsR* see NWMN_1665
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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