PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_RS14025 [old locus tag: NWMN_2441 ]
• pan locus tag^?: SAUPAN006199000
• symbol: NWMN_RS14025
• pan gene symbol^?: cidR
• synonym:
• product: LysR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_RS14025 [old locus tag: NWMN_2441 ]
• symbol: NWMN_RS14025
• product: LysR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 2683760..2684623
• length: 864
• essential: unknown other strains

⊟Accession numbers

• Location: NC_009641 (2683760..2684623) NCBI
• BioCyc:
• MicrobesOnline: see NWMN_2441

⊟Phenotype

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⊟DNA sequence

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  ATGAAATATTTTATTGAAGTCGTTAAGCAAGGAGGCATGACTAATGCTTCCAAATCATTA
  TATATTGCACAACCTACAATTAGTAAAGCAATTAAAGATATTGAAAATGAAATGGGCACG
  CCCTTATTTGATAGAAGTAAAAGACATTTAATTCTTACCGATGCAGGTCAAATTTTTTAT
  GAGAAAAGTAAAGAAATTGTTGCACTGTATGATTATTTACCATCTGAAATGGAACGCTTG
  AATGGACTGGAAACAGGACATATAAACATGGGCATGTCGGCAGTCATGAATATGAAGATT
  CTTATCAATATTCTTGGTGCATTCCATCAACAATATCCAAATGTTACATATAATTTGATA
  GAAAATGGCGGTAAAACAATTGAACAGCAAATTATCAATGATGAAGTAGATATAGGCGTG
  ACCACTTTGCCAGTCGATCATCATATTTTCGATTATACTACCCTAGATAAGGAAGATTTG
  CGACTTATCGTGAGCAGAGAGCATCGACTCGCAAAATATGAAACTGTTAAACTCGAAGAT
  TTAGCAGGTGAAGACTTCATTTTATTTAATAAAGACTTTTACTTGAATGATAAAATTATT
  GAAAATGCTAAAAACGTTGGCTTTGTTCCGAATACTGTAGCGCAAATTTCACAATGGCAT
  GTTATAGAAGATTTAGTTACGAATGAATTAGGTATTAGTATTTTACCAACATCAATTTCA
  GAGCAACTAAATGGAGATGTGAAGCTGCTACGCATTGAAGATGCTCATGTACATTGGGAA
  TTAGGTGTTGTTTGGAAGAAGGATAAACAATTAAGTCATGCCACAACGAAATGGATAGAA
  TTTTTGAAAGACCGTTTAGGCTAA
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⊟Protein

⊟General

• locus tag: NWMN_RS14025 [old locus tag: NWMN_2441 ]
• symbol: NWMN_RS14025
• description: LysR family transcriptional regulator
• length: 287
• theoretical pI: 5.22208
• theoretical MW: 32846.4
• GRAVY: -0.225784

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 131.4)
  and 8 more
  Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 82.9)
  Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 82.9)
  putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 53.7)
  Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 41.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 41.3)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 41.3)
  Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 33.9)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 15.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 143.8)
  and 2 more
  HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 64.4)
  no clan defined ERp29; Endoplasmic reticulum protein ERp29, C-terminal domain (PF07749; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007355
  • TAT(Tat/SPI): 0.000552
  • LIPO(Sec/SPII): 0.001499
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see NWMN_2441
• RefSeq: WP_001549809 NCBI
• UniProt: see NWMN_2441

⊟Protein sequence

• MKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMGTPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMNMKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTLDKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTVAQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQLSHATTKWIEFLKDRLG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SigB see NWMN_2441

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications