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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0249 [new locus tag: SA_RS01465 ]
  • pan locus tag?: SAUPAN001138000
  • symbol: scdA
  • pan gene symbol?: scdA
  • synonym:
  • product: cell wall biosynthesis protein ScdA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0249 [new locus tag: SA_RS01465 ]
  • symbol: scdA
  • product: cell wall biosynthesis protein ScdA
  • replicon: chromosome
  • strand: +
  • coordinates: 303583..304257
  • length: 675
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATAAATAAAAATGACATAGTAGCAGATATAGTAATAGATTATCCGAAAGCAGCAGAT
    ATTTTTAGAAGCGTAGGTATAGACTTTTGTTGCGGTGGACAAGTAAGTATAGAAGCAGCA
    TCCTTAGAAAAGAAAAATGTAGATTTGAACGAATTATTACAGCGTCTCAATGACGTTGAA
    CAAACGAATACACCAGGTTCGCTTAACCCTAAATTTTTAAATGTTTCGTCACTTATTCAA
    TATATTCAAGCAGCATATCATGAACCTCTTAGAGAAGAATTTAAAAATTTAACACCTTAT
    GTGACGAAATTATCGAAAGTACATGGACCTAACCATCCATATTTAGTCGAGTTAAAAGAA
    ACATATGATACATTTAAAAGTGGCATGTTAGAGCATATGCAAAAAGAAGATGATGTTGAT
    TTTCCAAAACTAATTAAATATGAACAAGGTGAAGTAGTAAACGATATTAATACAGTGATT
    GATGATTTAGTATCTGATCACATTGCAACGGGACAATTGTTAGTGAAAATGAGCGATTTA
    ACATCTAGCTATGAACCACCGATAGAGGCATGTGGTACGTGGCGACTCGTTTATCAGAGA
    TTAAAAGCACTTGAAGTGTTAACACATGAGCATGTTCATTTAGAGAATCATGTTTTATTT
    AAAAAAGTATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0249 [new locus tag: SA_RS01465 ]
  • symbol: ScdA
  • description: cell wall biosynthesis protein ScdA
  • length: 224
  • theoretical pI: 4.99337
  • theoretical MW: 25467.8
  • GRAVY: -0.290179

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster repair di-iron protein (TIGR03652; HMM-score: 254.7)
    and 2 more
    Metabolism Energy metabolism Electron transport hybrid cluster protein-associated redox disulfide domain (TIGR03980; HMM-score: 14.3)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 13.1)
  • TheSEED  :
    • Repair of Iron Centers di-iron protein
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Nitrosative stress  Nitric oxide-dependent regulator DnrN or NorA
    and 1 more
    Stress Response Stress Response - no subcategory Flavohaemoglobin  Nitric oxide-dependent regulator DnrN or NorA
  • PFAM:
    no clan defined ScdA_N; Domain of Unknown function (DUF542) (PF04405; HMM-score: 74.1)
    Hemerythrin; Hemerythrin HHE cation binding domain (PF01814; HMM-score: 68.2)
    and 1 more
    DUF1858; Domain of unknown function (DUF1858) (PF08984; HMM-score: 19.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01877
    • TAT(Tat/SPI): 0.000848
    • LIPO(Sec/SPII): 0.00485
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINKNDIVADIVIDYPKAADIFRSVGIDFCCGGQVSIEAASLEKKNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVTKLSKVHGPNHPYLVELKETYDTFKSGMLEHMQKEDDVDFPKLIKYEQGEVVNDINTVIDDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHVLFKKVS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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