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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0255 [new locus tag: SA_RS01495 ]
  • pan locus tag?: SAUPAN001145000
  • symbol: SA0255
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0255 [new locus tag: SA_RS01495 ]
  • symbol: SA0255
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 309210..310001
  • length: 792
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAGCAATAAATATAAAGAACAAGCCCAAGACATTCTTACAGCTGTAGGTGGTGTCGAA
    AACATTGTTGATGCAACGTATGATACGAAGTGCATTACAATTCATATGCAACATACAATT
    CCTTCTACAGCAAATGAAGTGAAACAAATAGTTGATGTGACATCTGTAGCAGAAAATGAT
    GCGCAGTTAGTCATAAAATTAAATGGAAATGTCGATGAAGTGTATCAGCAATTACAGCGA
    TTAATTAAGAATGCTAATGTCGAAGAGAGTGAGAATACTGACAATATTAATAGCCAAGAT
    ACAAGTTATACACCTCAAGTAAAAGTAACAACACCAATTTTAGTGAAAGCACCAATCGCT
    GGTCGTCGTATTTTACTTAAAGAAGTAAGAGATTCAATTTTTAGAGAGAAAATGGTAGGT
    GAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACCGTTCAATGGTTTA
    ATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGAC
    ATAGTCATTCATATTGGCGTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTT
    GTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAATTCGACCAGCAATAT
    ATACAACAACAAGGCTACAATGCTGACGTTATTGTCGTTATTAGCAACTCTGCCGATTTA
    GGAAAAGTAGAACTGACAATGAATGAAATCATTACGACTGAAGATGTTATTTTTAAAATA
    TTTAAAAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    792

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0255 [new locus tag: SA_RS01495 ]
  • symbol: SA0255
  • description: hypothetical protein
  • length: 263
  • theoretical pI: 4.71001
  • theoretical MW: 29106.9
  • GRAVY: -0.171103

⊟Function[edit | edit source]

  • TIGRFAM:
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 170.5)
    and 6 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 129.4)
    Signal transduction Signal transduction PTS PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 129.4)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 29)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 9.3)
    Signal transduction Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 9.3)
  • TheSEED  :
    • PTS system, N-acetylglucosamine-specific IIA component (EC 2.7.1.193)
    • PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.193)
    • PTS system, N-acetylglucosamine-specific IIC component
    Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  PTS system, N-acetylglucosamine-specific IIA component
    and 5 more
    Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69)
    Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  PTS system, N-acetylglucosamine-specific IIC component
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  PTS system, N-acetylglucosamine-specific IIA component
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  PTS system, N-acetylglucosamine-specific IIC component
  • PFAM:
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 150.8)
    and 5 more
    Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 23.4)
    no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 15.6)
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 14.8)
    no clan defined TPD; Protein of unknown function TPD sequence-motif (PF14811; HMM-score: 12.9)
    Hybrid (CL0105) RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018393
    • TAT(Tat/SPI): 0.000523
    • LIPO(Sec/SPII): 0.001626
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTANEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTSYTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGLISMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQQYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BglR* (repression) regulon
    BglR*(TF)important in Beta-glucosides utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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