PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0259 [new locus tag: SAUSA300_RS01380 ]
• pan locus tag^?: SAUPAN001145000
• symbol: SAUSA300_0259
• pan gene symbol^?: —
• synonym:
• product: PTS system, IIA component

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0259 [new locus tag: SAUSA300_RS01380 ]
• symbol: SAUSA300_0259
• product: PTS system, IIA component
• replicon: chromosome
• strand: +
• coordinates: 310809..311600
• length: 792
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913661 NCBI
• RefSeq: YP_492973 NCBI
• BioCyc: see SAUSA300_RS01380
• MicrobesOnline: 1291774 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAGCAATAAATATAAAGAACAAGCCCAAGACATTCTTACAGCTGTAGGTGGTGTCGAA
  AACATTGTTGATGCAACGTATGATACGAAGTGCATTACAATTCATATGCAACATACAATT
  CCTTCTACAGCAAATGAAGTGAAACAAATAGTTGATGTGACATCTGTAGCAGAAAATGAT
  ACGCAGTTAGTCATAAAATTAAATGGAAATGTCGATGAAGTGTATCAGCAATTACAGCGA
  TTAATTAAGAATGCTAATGTCGAAGAGAGTGAGAATACTGACAATATTAATAGTCAAGAT
  ACAAGTTATACACCTCAAGTAAAAGTAACAACACCAATTTTAGTGAAAGCACCAATCGCT
  GGTCGTCGTATTTTACTTAAAGAAGTAAGAGATTCAATTTTTAGAGAGAAAATGGTAGGT
  GAAGGCTTAGCAATCAAAGCTCATGAAGAATCCAAAGTAATCGCACCGTTCAATGGTTTA
  ATATCTATGATTGTACCAACTAAGCATGCAGTTGGTATTCAATCAGAAGACGGTGTGGAC
  ATAGTCATTCATATTGGCTTGAATACAGTTGACTTGGAAGGTAAAGGGTTCAAGTGCTTT
  GTAAAGCAAAATGATCATGTTGAAGCAGGGCAAACGTTGTTGCAATTCGACCAGCAATAT
  ATACAACAACAAGGCTACAATGCTGACGTTATTGTCGTTATTAGCAACTCTGCCGATTTA
  GGAAAAGTAGAACTGACAATGAATGAAATCATTACGACTGAAGATGTTATTTTTAAAATA
  TTTAAAAACTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_0259 [new locus tag: SAUSA300_RS01380 ]
• symbol: SAUSA300_0259
• description: PTS system, IIA component
• length: 263
• theoretical pI: 4.71001
• theoretical MW: 29150.9
• GRAVY: -0.182129

⊟Function

• reaction:
  EC 2.7.1.69?  ExPASy
  Transferred entry: 2.7.1.191, 2.7.1.192, 2.7.1.193, 2.7.1.194, 2.7.1.195, 2.7.1.196, 2.7.1.197, 2.7.1.198, 2.7.1.199, 2.7.1.200, 2.7.1.201, 2.7.1.202, 2.7.1.203, 2.7.1.204, 2.7.1.205, 2.7.1.206, 2.7.1.207 and 2.7.1.208
• TIGRFAM:
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 170.9)
  and 4 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 130)
  Signal transduction Signal transduction PTS PTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 130)
  PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 29)
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 28.5)
• TheSEED  :
  • PTS system, N-acetylglucosamine-specific IIA component
  • PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69)
  • PTS system, N-acetylglucosamine-specific IIC component
  Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  PTS system, N-acetylglucosamine-specific IIA component
  and 5 more
  Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69)
  Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  PTS system, N-acetylglucosamine-specific IIC component
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  PTS system, N-acetylglucosamine-specific IIA component
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  PTS system, N-acetylglucosamine-specific IIC component
• PFAM:
  Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 151.4)
  and 5 more
  Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 23.4)
  no clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 15.6)
  Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 15.1)
  no clan defined TPD; Protein of unknown function TPD sequence-motif (PF14811; HMM-score: 12.9)
  Hybrid (CL0105) RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.019603
  • TAT(Tat/SPI): 0.000565
  • LIPO(Sec/SPII): 0.001731
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160259 NCBI
• RefSeq: YP_492973 NCBI
• UniProt: A0A0H2XE58 UniProt

⊟Protein sequence

• MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTANEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTSYTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGLISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQQYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0259 > bglA

⊟Regulation

• regulator: BglR* (repression) regulon

  BglR*

  (TF)

  important in Beta-glucosides utilization; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications