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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0393 [new locus tag: SA_RS02255 ]
  • pan locus tag?: SAUPAN002129000
  • symbol: set15
  • pan gene symbol?: ssl11
  • synonym:
  • product: superantigen-like protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0393 [new locus tag: SA_RS02255 ]
  • symbol: set15
  • product: superantigen-like protein
  • replicon: chromosome
  • strand: +
  • coordinates: 454143..454826
  • length: 684
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAATTAAAAAATATTGCTAAAGCAAGTTTAGCACTAGGGATTTTAACAACAGGTATG
    ATTACAACTACTGCTCAGCCAGTAAAAGCAAGTACATTAGAGGTTAGATCACAAGCTACT
    CAAGACTTGAGTGAATATTATAAAGGGAGAGGATTTGAATTAACAAATGTAACAGGATAT
    AAATACGGAAATAAAGTTACATTTATAGATAATTCTCAACAAATTGATGTTACATTGACA
    GGAAATGAAAAATTAACTGTTAAAGATGATGACGAAGTTTCTAATGTTGACGTGTTTGTA
    GTAAGAGAAGGTAGTGACAAATCAGCTATCACAACATCGATTGGTGGAATTACAAAGACA
    AATGGGACTCAACATAAAGATACTGTTCAAAACGTTAATTTGTCAGTTTCTAAGAGTACA
    GGTCAACACACTACTTCTGTGACTTCAGAATACTATAGCATCTATAAAGAAGAAATCTCA
    TTAAAAGAACTTGATTTTAAATTAAGAAAGCATTTAATTGATAAACATGATCTTTATAAG
    ACAGAACCTAAAGACAGTAAAATTAGAATTACTATGAAAAACGGTGGCTATTATACATTT
    GAATTAAATAAAAAATTACAGCCTCATCGCATGGGTGATACGATTGATAGTAGAAATATA
    GAGAAAATTGAAGTGAATTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    684

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0393 [new locus tag: SA_RS02255 ]
  • symbol: Set15
  • description: superantigen-like protein
  • length: 227
  • theoretical pI: 9.3074
  • theoretical MW: 25439.5
  • GRAVY: -0.611454

⊟Function[edit | edit source]

  • TIGRFAM:
    phospholamban (TIGR01294; HMM-score: 11.2)
  • TheSEED  :
    • Phage exotoxin
  • PFAM:
    OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 69.5)
    and 3 more
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 27.3)
    no clan defined DUF5322; Family of unknown function (DUF5322) (PF17255; HMM-score: 13.1)
    GT-C (CL0111) PTPS_related; 6-pyruvoyl-tetrahydropterin synthase related domain; membrane protein (PF10131; HMM-score: 9.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.988485
    • TAT(Tat/SPI): 0.007632
    • LIPO(Sec/SPII): 0.00239
    • Cleavage Site: CS pos: 30-31. VKA-ST. Pr: 0.8556
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLSEYYKGRGFELTNVTGYKYGNKVTFIDNSQQIDVTLTGNEKLTVKDDDEVSNVDVFVVREGSDKSAITTSIGGITKTNGTQHKDTVQNVNLSVSKSTGQHTTSVTSEYYSIYKEEISLKELDFKLRKHLIDKHDLYKTEPKDSKIRITMKNGGYYTFELNKKLQPHRMGDTIDSRNIEKIEVNL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)

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