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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0987 [new locus tag: SA_RS05595 ]
  • pan locus tag?: SAUPAN003376000
  • symbol: SA0987
  • pan gene symbol?: rnhC
  • synonym:
  • product: ribonuclease HIII

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0987 [new locus tag: SA_RS05595 ]
  • symbol: SA0987
  • product: ribonuclease HIII
  • replicon: chromosome
  • strand: -
  • coordinates: 1118205..1119143
  • length: 939
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGAATATCGTTTTTAAATTGTCGGATAAAGACATAACGACATTAATGTCACGCATT
    ACTTTTGATACTGAGAATTTACCTCAAGGTATGAAAGCACGTGCAAAGTATCAAAATACA
    ACTGTAAATATTTACCAATCAGGTAAAGTCATGTTTCAAGGTAATCATGCAGAAGCTGTG
    TCTAAAGAACTTCTACCGCAGCATTCTCAATTGAATACTAATAAAACTAAAAAGAAAAAT
    ATGGCTAATTCTTCTCTTGAACAAACTTTAATGTATGATCAATTTAACTGTATTGGTAGT
    GATGAGGCTGGAAGTGGCGATTACTTTGGTCCTTTGACTGTGTGTGCTGCTTTTGTTACC
    AAAGAGCATGTTCCTATTTTAAAAACTTTGGGTGTAGATGATTCAAAAAAATTAACCGAT
    ACCAAAATTGTAGAACTTGCTGAGCAATTGGTTGCTTTCATCCCACATTCACTATTAACA
    CTACACAATGATAAATATAATATTCAGCAAGCAAAAGGCTGGACTCAAGTAAAAATGAAA
    GCTGTTCTTCATAATGAAGCCATCAAAAATGTACTTGAAAAAATTGATTCGTCTCAATTA
    GACTATATCGTCATTGATCAGTTCGCAAAACGCGAAGTGTATAGCCATTACGCATTATCC
    GATATACCACTTCCAAAGAAAACAAAATTTGAAACAAAAGGAGAATCGAAGTCATTAGCC
    ATTGCAGTGGCAAGCATAATTTCTAGATACGCATTTATTACATATATGGATCAAATATCA
    AAATATATTAATATGACGATTCCTAAAGGTGCCGGTGCTAAAGTCGATGTAATTGCCGCA
    AAAATTATAAAAAAATACGGTCTTTCACGTTTAGATACCATTTCAAAAAAGCATTTTAAA
    AATCGTGAAAAAGCCCAAAAAATTTTAAAGCCTCTTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0987 [new locus tag: SA_RS05595 ]
  • symbol: SA0987
  • description: ribonuclease HIII
  • length: 312
  • theoretical pI: 10.0243
  • theoretical MW: 35054.4
  • GRAVY: -0.322115

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.26.4?  ExPASy
    Ribonuclease H Endonucleolytic cleavage to 5'-phosphomonoester
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ribonuclease HIII (TIGR00716; EC 3.1.26.4; HMM-score: 226.7)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ribonuclease HII (TIGR00729; EC 3.1.26.4; HMM-score: 60.7)
    Metabolism Energy metabolism Electron transport ubiquinol-cytochrome c reductase, iron-sulfur subunit (TIGR01416; EC 1.10.2.2; HMM-score: 10.5)
  • TheSEED  :
    • Ribonuclease HIII (EC 3.1.26.4)
    RNA Metabolism RNA processing and modification Ribonuclease H  Ribonuclease HIII (EC 3.1.26.4)
  • PFAM:
    RNase_H (CL0219) RNase_HII; Ribonuclease HII (PF01351; HMM-score: 157.2)
    and 2 more
    TBP-like (CL0407) DUF3378; Domain of unknown function (DUF3378) (PF11858; HMM-score: 74)
    TBP; Transcription factor TFIID (or TATA-binding protein, TBP) (PF00352; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012776
    • TAT(Tat/SPI): 0.000911
    • LIPO(Sec/SPII): 0.001623
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNIYQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSSLEQTLMYDQFNCIGSDEAGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVAFIPHSLLTLHNDKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYALSDIPLPKKTKFETKGESKSLAIAVASIISRYAFITYMDQISKYINMTIPKGAGAKVDVIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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