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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1150 [new locus tag: SACOL_RS05875 ]
  • pan locus tag?: SAUPAN003376000
  • symbol: rnhC
  • pan gene symbol?: rnhC
  • synonym:
  • product: ribonuclease HIII

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1150 [new locus tag: SACOL_RS05875 ]
  • symbol: rnhC
  • product: ribonuclease HIII
  • replicon: chromosome
  • strand: -
  • coordinates: 1159176..1160114
  • length: 939
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCGAATATCGTTTTTAAATTGTCGGATAAAGACATAACGACATTAATGTCACGCATT
    TCTTTTGATACTGAGAATTTACCTCAAGGTATGAAAGCACGTGCAAAGTATCAAAATACA
    ACTGTAAATATTTACCAATCAGGTAAAGTCATGTTTCAAGGTAATCATGCAGAAGCTGTG
    TCTGAAGAACTTCTACCGCAGCATTCTCAATTGAATACTAATAAAACTAAAAAGAAAAAT
    ATGGCTAATTCTTTTCTTGAACAAACTTTAATGTATGATCAATTTAACTGTATTGGTAGT
    GATGAGGCTGGAAGTGGCGATTACTTTGGTCCGTTGACTGTGTGTGCTGCTTTCGTTACC
    AAAGAGCATGTCCCTATTTTAAAAACTTTGGGTGTAGATGATTCAAAAAAATTAACCGAT
    ACCAAAATTGTAGAACTTGCTGAGCAATTGGTTACTTTCATACCACATTCATTATTAACA
    CTACACAATGAAAAATATAATATTCAGCAAGCAAAAGGCTGGACTCAAGTAAAAATGAAA
    GCTGCCCTTCATAATGAAGCCATCAAAAATGTACTTGAAAAAATTGATTCGTCTCAATTA
    GACTATATCGTCATTGATCAGTTCGCAAAACGCGAAGTGTATAGCCATTACGCATTATCA
    GATATACCACTTCCAAAGAAAACAAAATTTGAAACAAAAGGAGAATCGAAGTCATTAGCC
    ATTGCAGTGGCAAGCATAATTTCTCGATACGCATTTGTTACATATATGGATCAAATATCA
    AAAAATATTAATATGACGATTCCTAAAGGTGCCGGTGCTAAAGTCGATGTAATCGCCGCC
    AAAATTATAAAAAAATACGGTCTTTCACGTTTAGATACCATTTCAAAAAAGCATTTTAAA
    AATCGTGAAAAAGCCCAAAAAATTTTAAAGCCTCTTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1150 [new locus tag: SACOL_RS05875 ]
  • symbol: RnhC
  • description: ribonuclease HIII
  • length: 312
  • theoretical pI: 9.93953
  • theoretical MW: 35054.3
  • GRAVY: -0.333333

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.26.4?  ExPASy
    Ribonuclease H Endonucleolytic cleavage to 5'-phosphomonoester
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ribonuclease HIII (TIGR00716; EC 3.1.26.4; HMM-score: 230.5)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ribonuclease HII (TIGR00729; EC 3.1.26.4; HMM-score: 63)
  • TheSEED  :
    • Ribonuclease HIII (EC 3.1.26.4)
    RNA Metabolism RNA processing and modification Ribonuclease H  Ribonuclease HIII (EC 3.1.26.4)
  • PFAM:
    RNase_H (CL0219) RNase_HII; Ribonuclease HII (PF01351; HMM-score: 162.4)
    and 3 more
    TBP-like (CL0407) DUF3378; Domain of unknown function (DUF3378) (PF11858; HMM-score: 75.4)
    TBP; Transcription factor TFIID (or TATA-binding protein, TBP) (PF00352; HMM-score: 13.7)
    no clan defined DUF1160; Protein of unknown function (DUF1160) (PF06648; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010962
    • TAT(Tat/SPI): 0.00101
    • LIPO(Sec/SPII): 0.001276
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNIYQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDEAGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLTLHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYALSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVDVIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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