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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1725 [new locus tag: SA_RS09900 ]
  • pan locus tag?: SAUPAN004939000
  • symbol: SA1725
  • pan gene symbol?: scpA
  • synonym:
  • product: staphopain, cysteine proteinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1725 [new locus tag: SA_RS09900 ]
  • symbol: SA1725
  • product: staphopain, cysteine proteinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1976121..1977287
  • length: 1167
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAAGAAACTTTCCAAAATTAATAGCTTTAAGTTTAATATTTTCTTTAAGTGTTACC
    CCTATTGCAAACGCTGAGAGCAATTCAAATATCAAAGCAAAAGATAAGAAGCATGTGCAA
    GTTAATGTCGAGGACAAGAGTGTTCCTACTGACGTACGTAATTTAGCGCAAAAAGACTAT
    CTTTCTTATGTTACTTCACTTGATAAAATCTATAATAAAGAGAAAGCCTCGTATACACTT
    GGTGAACCTTTTAAAATTTATAAATTCAACAAAAAGAGTGATGGTAATTATTATTTTCCA
    GTTTTGAACACTGAAGGTAATATCGATTATATAGTTACAATTTCACCAAAAATCACTAAG
    TATTCTAGTTCATCTTCTAAATACACAATCAATGTTTCTCCATTTTTATCTAAAGTCTTA
    AATCAATATAAAGATCAACAAATTACTATTTTGACAAACTCAAAAGGTTACTATGTGGTT
    ACTCAAAATCATAAAGCTAAACTAGTACTAAAAACACCACGTCTAGAAGATAAAAAGTTA
    AAAAAGACTGAGAGTATACCTACTGGCAATAATGTAACGCAACTAAAGCAAAAAGCTTCT
    GTCACTATGCCAACTAGTCAATTTAAAAGTAATAATTATACTTACAACGAGCAATATATA
    AATAAACTTGAGAATTTCAAGATAAGAGAAACTCAAGGTAACAATGGTTGGTGTGCAGGC
    TATACGATGTCTGCATTACTAAATGCGACTTATAATACTAACAAATATCATGCAGAAGCT
    GTGATGAGATTTTTACACCCAAACTTACAAGGACAACGATTCCAATTTACTGGTTTAACG
    CCAAGAGAGATGATTTATTTTGGACAAACTCAGGGAAGAAGTCCACAACTTCTTAATAGA
    ATGACTACATATAATGAAGTTGACAATCTTACTAAAAATAATAAAGGAATTGCTGTTTTA
    GGTAGTAGAGTAGAATCAAGAAATGGTATGCACGCAGGGCATGCCATGGCAGTTGTTGGG
    AACGCTAAATTAGATAATGGACAAGAGGTTATAATTATATGGAATCCATGGGATAATGGT
    TTTATGACTCAAGATGCTAAAAACAATGTTATTCCAGTATCCAATGGCGACCATTATCGA
    TGGTATTCATCTATTTATGGTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1167

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1725 [new locus tag: SA_RS09900 ]
  • symbol: SA1725
  • description: staphopain, cysteine proteinase
  • length: 388
  • theoretical pI: 10.107
  • theoretical MW: 44203.9
  • GRAVY: -0.62835

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.22.48?  ExPASy
    Staphopain Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate
  • TIGRFAM:
    Genetic information processing Transcription RNA processing ribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 10)
  • TheSEED  :
    • Staphopain A precursor (EC 3.4.22.48)
  • PFAM:
    Peptidase_CA (CL0125) Peptidase_C47; Staphopain peptidase C47 (PF05543; HMM-score: 335.7)
    Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 284.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: IANAESNS
  • SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.994703
    • TAT(Tat/SPI): 0.001864
    • LIPO(Sec/SPII): 0.002483
    • Cleavage Site: CS pos: 25-26. ANA-ES. Pr: 0.9149
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENFKIRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Renata Filipek, Roman Szczepanowski, Artur Sabat, Jan Potempa, Matthias Bochtler
Prostaphopain B structure: a comparison of proregion-mediated and staphostatin-mediated protease inhibition.
Biochemistry: 2004, 43(44);14306-15
[PubMed:15518582] [WorldCat.org] [DOI] (P p)

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