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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02127
  • pan locus tag?: SAUPAN004939000
  • symbol: SAOUHSC_02127
  • pan gene symbol?: scpA
  • synonym:
  • product: staphopain thiol proteinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02127
  • symbol: SAOUHSC_02127
  • product: staphopain thiol proteinase
  • replicon: chromosome
  • strand: +
  • coordinates: 2002339..2003505
  • length: 1167
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAAGAAACTTTCCAAAATTAATAGCTTTAAGTTTAATATTTTCTTTAAGTGTTACC
    CCTATTGCAAACGCTGAGAGCAATTCAAATATCAAAGCAAAAGATAAGAAGCATGTGCAA
    GTTAATGTCGAGGACAAGAGTGTTCCTACTGACGTACGTAATTTAGCGCAAAAAGACTAT
    CTTTCTTATGTTACTTCACTTGATAAAATCTATAATAAAGAGAAAGCCTCGTATACACTT
    GGTGAACCTTTTAAAATTTATAAATTCAACAAAAAGAGTGATGGTAATTATTATTTTCCA
    GTTTTGAACACTGAAGGTAATATCGATTATATAGTTACAATTTCACCAAAAATCACTAAG
    TATTCTAGTTCATCTTCTAAATACACAATCAATGTTTCTCCATTTTTATCTAAAGTCTTA
    AATCAATATAAAGATCAACAAATTACTATTTTGACAAACTCAAAAGGTTACTATGTGGTT
    ACTCAAAATCATAAAGCTAAACTAGTACTAAAAACACCACGTCTAGAAGATAAAAAGTTA
    AAAAAGACTGAGAGTATACCTACTGGCAATAATGTAACGCAACTAAAGCAAAAAGCTTCT
    GTCACTATGCCAACTAGTCAATTTAAAAGTAATAATTATACTTACAACGAGCAATATATA
    AATAAACTTGAGAATTTCAAGATAAGAGAAACTCAAGGTAACAATGGTTGGTGTGCAGGC
    TATACGATGTCTGAATTACTAAATGCGACTTATAATACTAACAAATATCATGCAGAAGCT
    GTGATGAGATTTTTACACCCAAACTTACAAGGACAACGATTCCAATTTACTGGTTTAACG
    CCAAGAGAGATGATTTATTTTGGACAAACTCAGGGAAGAAGTCCACAACTTCTTAATAGA
    ATGACTACATATAATGAAGTTGACAATCTTACTAAAAATAATAAAGGAATTGCTGTTTTA
    GGTAGTAGAGTAGAATCAAGAAATGGTATGCACGCAGGGCATGCCATGGCAGTTGTTGGG
    AACGCTAAATTAGATAATGGACAAGAGGTTATAATTATATGGAATCCATGGGATAATGGT
    TTTATGACTCAAGATGCTAAAAACAATGTTATTCCAGTATCCAATGGCGACCATTATCGA
    TGGTATTCATCTATTTATGGTTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1167

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02127
  • symbol: SAOUHSC_02127
  • description: staphopain thiol proteinase
  • length: 388
  • theoretical pI: 10.0693
  • theoretical MW: 44261.9
  • GRAVY: -0.64201

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.22.48?  ExPASy
    Staphopain Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate
  • TIGRFAM:
    Genetic information processing Transcription RNA processing ribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 10.2)
  • TheSEED  :
    • Staphopain A precursor (EC 3.4.22.48)
  • PFAM:
    Peptidase_CA (CL0125) Peptidase_C47; Staphopain peptidase C47 (PF05543; HMM-score: 335.2)
    Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 284.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: IANAESNS
  • SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.994703
    • TAT(Tat/SPI): 0.001864
    • LIPO(Sec/SPII): 0.002483
    • Cleavage Site: CS pos: 25-26. ANA-ES. Pr: 0.9149
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Lindsey Shaw, Ewa Golonka, Jan Potempa, Simon J Foster
The role and regulation of the extracellular proteases of Staphylococcus aureus.
Microbiology (Reading): 2004, 150(Pt 1);217-228
[PubMed:14702415] [WorldCat.org] [DOI] (P p)

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