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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1847 [new locus tag: NWMN_RS10610 ]
- pan locus tag?: SAUPAN004939000
- symbol: sspB
- pan gene symbol?: scpA
- synonym:
- product: staphopain thiol proteinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1847 [new locus tag: NWMN_RS10610 ]
- symbol: sspB
- product: staphopain thiol proteinase
- replicon: chromosome
- strand: +
- coordinates: 2058643..2059809
- length: 1167
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5332301 NCBI
- RefSeq: YP_001332881 NCBI
- BioCyc:
- MicrobesOnline: 3707435 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGAAAAGAAACTTTCCAAAATTAATAGCTTTAAGTTTAATATTTTCTTTAAGTGTTACC
CCTATTGCAAACGCTGAGAGCAATTCAAATATCAAAGCAAAAGATAAGAAGCATGTGCAA
GTTAATGTCGAGGACAAGAGTGTTCCTACTGACGTACGTAATTTAGCGCAAAAAGACTAT
CTTTCTTATGTTACTTCACTTGATAAAATCTATAATAAAGAGAAAGCCTCGTATACACTT
GGTGAACCTTTTAAAATTTATAAATTCAACAAAAAGAGTGATGGTAATTATTATTTTCCA
GTTTTGAACACTGAAGGTAATATCGATTATATAGTTACAATTTCACCAAAAATCACTAAG
TATTCTAGTTCATCTTCTAAATACACAATCAATGTTTCTCCATTTTTATCTAAAGTCTTA
AATCAATATAAAGATCAACAAATTACTATTTTGACAAACTCAAAAGGTTACTATGTGGTT
ACTCAAAATCATAAAGCTAAACTAGTACTAAAAACACCACGTCTAGAAGATAAAAAGTTA
AAAAAGACTGAGAGTATACCTACTGGCAATAATGTAACGCAACTAAAGCAAAAAGCTTCT
GTCACTATGCCAACTAGTCAATTTAAAAGTAATAATTATACTTACAACGAGCAATATATA
AATAAACTTGAGAATTTCAAGATAAGAGAAACTCAAGGTAACAATGGTTGGTGTGCAGGC
TATACGATGTCTGAATTACTAAATGCGACTTATAATACTAACAAATATCATGCAGAAGCT
GTGATGAGATTTTTACACCCAAACTTACAAGGACAACGATTCCAATTTACTGGTTTAACG
CCAAGAGAGATGATTTATTTTGGACAAACTCAGGGAAGAAGTCCACAACTTCTTAATAGA
ATGACTACATATAATGAAGTTGACAATCTTACTAAAAATAATAAAGGAATTGCTGTTTTA
GGTAGTAGAGTAGAATCAAGAAATGGTATGCACGCAGGGCATGCCATGGCAGTTGTTGGG
AACGCTAAATTAGATAATGGACAAGAGGTTATAATTATATGGAATTCATGGGATAATGGT
TTTATGACTCAAGATGCTAAAAACAATGTTATTCCAGTATCCAATGGCGACCATTATCGA
TGGTATTCATCTATTTATGGTTATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1847 [new locus tag: NWMN_RS10610 ]
- symbol: SspB
- description: staphopain thiol proteinase
- length: 388
- theoretical pI: 10.0693
- theoretical MW: 44251.9
- GRAVY: -0.639948
⊟Function[edit | edit source]
- TIGRFAM: Transcription RNA processing ribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 10.2)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: Peptidase_CA (CL0125) Peptidase_C47; Staphopain peptidase C47 (PF05543; HMM-score: 330.8)Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 284.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.02
- Extracellular Score: 9.98
- Internal Helices: 0
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: -0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: -2
- Predicted Cleavage Site: IANAESNS
- SignalP: Signal peptide SP(Sec/SPI) length 25 aa
- SP(Sec/SPI): 0.994703
- TAT(Tat/SPI): 0.001864
- LIPO(Sec/SPII): 0.002483
- Cleavage Site: CS pos: 25-26. ANA-ES. Pr: 0.9149
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKRNFPKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENFKIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQEVIIIWNSWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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