PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2178 [new locus tag: SA_RS12500 ]
• pan locus tag^?: SAUPAN005920000
• symbol: SA2178
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2178 [new locus tag: SA_RS12500 ]
• symbol: SA2178
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2446722..2447462
• length: 741
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125106 NCBI
• RefSeq: NP_375501 NCBI
• BioCyc: see SA_RS12500
• MicrobesOnline: 104527 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGGCATACACATACACTTTAAAAGATATTATTGAAATTACAGGTGTAACTAAAAGAACT
  TTACATTATTACGATGAAATAGGATTATTAGTTCCAGATAAAAATGATAAAAATTATCGC
  GTTTATAAACAGCAAGACTTAGAAAAATTACAAAAGATTTTAATACTCAAGTCTTTTGAT
  TTTGATATCGCTAAAATAAAACAATACATTTCGTATGATAATGAACAATTGCGAAAGTTA
  TTATCAGAGCAAATAAGCAAGTTAGATAAAAAGATTTCTGACTTACAATTAATTAGACGC
  TCTGTATGTGAATTTATTAAAGGACTCTCTCTAATAGATACCAGCATTTTAAACAAGACA
  CTACAGTCACAATATGATAAAGAAGCATCTATAAAATATGGTCATACGAAAGCATATCAA
  TCATTTATTAGACGTAAAGACAGCTTACAATCGCAGGATATCAGACATAAATTGACAACT
  ATCTTCAATAAATTTAATCATATGTCTTTGAGTCATTATCCAATCCAAGATTGTAGTGAT
  CTCGTATTTGAGTGGAAGGCATTTATGAACACTATCGCTGATTTTGATGATGAAACATTA
  TGCTGTATTGCTAAAACATATGAAGATGATACGCGTTTCAAAGATTACTTTAATTCATAT
  GATAATCAAAATTTAGCATCATACATTTCAGAAGCTGTTAATTATTTTTTGAGCAATGTG
  AATAAGAGCGACAATTTTTAA
  60
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  741
  

⊟Protein

⊟General

• locus tag: SA2178 [new locus tag: SA_RS12500 ]
• symbol: SA2178
• description: hypothetical protein
• length: 246
• theoretical pI: 7.48621
• theoretical MW: 29126.9
• GRAVY: -0.555285

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions Cu(I)-responsive transcriptional regulator (TIGR02044; HMM-score: 44.6)
  Signal transduction Regulatory functions DNA interactions Cd(II)/Pb(II)-responsive transcriptional regulator (TIGR02047; HMM-score: 40.5)
  Cellular processes Cellular processes Detoxification Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 39.6)
  Signal transduction Regulatory functions DNA interactions Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 39.6)
  Signal transduction Regulatory functions DNA interactions Zn(II)-responsive transcriptional regulator (TIGR02043; HMM-score: 36.7)
  and 2 more
  Cellular processes Cellular processes Detoxification redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 21.3)
  Signal transduction Regulatory functions DNA interactions redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 21.3)
• TheSEED  :
  • Transcriptional regulator, MerR family
• PFAM:
  HTH (CL0123) MerR_1; MerR HTH family regulatory protein (PF13411; HMM-score: 60.7)
  no clan defined TipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 53.8)
  and 6 more
  HTH (CL0123) MerR; MerR family regulatory protein (PF00376; HMM-score: 42.3)
  MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 23.1)
  no clan defined MKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 14.6)
  FliD_N; Flagellar hook-associated protein 2 N-terminus (PF02465; HMM-score: 12.5)
  Ribosomal_L12_N; Ribosomal protein L7/L12 dimerisation domain (PF16320; HMM-score: 12.5)
  HTH (CL0123) MerR_2; MerR HTH family regulatory protein (PF13591; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.0016
  • TAT(Tat/SPI): 0.00007
  • LIPO(Sec/SPII): 0.000362
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927968 NCBI
• RefSeq: NP_375501 NCBI
• UniProt: A0A0H3JML4 UniProt

⊟Protein sequence

• MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQQDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSVCEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTTIFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFNSYDNQNLASYISEAVNYFLSNVNKSDNF

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2177 > SA2178

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications