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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2388 [new locus tag: SACOL_RS12530 ]
  • pan locus tag?: SAUPAN005920000
  • symbol: SACOL2388
  • pan gene symbol?: —
  • synonym:
  • product: MerR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2388 [new locus tag: SACOL_RS12530 ]
  • symbol: SACOL2388
  • product: MerR family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 2447234..2447974
  • length: 741
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    TTGGCATACACATACACTTTAAAAGATATTATTGAAATTACAGGTGTAACTAAAAGAACT
    TTACATTATTACGATGAAATAGGATTATTAGTTCCAGATAAAAATGATAAAAATTATCGC
    GTTTATAAACAGCAAGACTTAGAAAAATTACAAAAGATTTTAATACTCAAGTCTTTTGAT
    TTTGATATCGCTAAAATAAAACAATACATTTCGTATGATAATGAACAATTGCGAAAGTTA
    TTATCAGAGCAAATAAGCAAGTTAGATAAAAAGATTTCTGACTTACAATTAATTAGACGC
    TCTGTATGTGAATTTATTAAAGGACTCTCTCTAATAGATACCAGCATTTTAAACAAGACA
    CTACAGTCACAATATGATAAAGAAGCATCTATAAAATATGGTCATACGAAAGCATATCAA
    TCATTTATTAGACGTAAAGACAGCTTACAATCGCAGGATATCAGACATAAATTGACAACT
    ATCTTCAATAAATTTAATCATATGTCTTTGAGTCATTATCCAATCCAAGATTGTAGTGAT
    CTCGTATTTGAGTGGAAGGCATTTATGAACACTATCGCTGATTTTGATGATGAAACATTA
    TGCTGTATTGCTAAAACATATGAAGATGATACGCGTTTCAAAGATTACTTTAATTCATAT
    GATAATCAAAATTTAGCATCATACATTTCAGAAGCTGTTAATTATTTTTTGAGCAATGTG
    AATAAGAGCGACAATTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    741

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2388 [new locus tag: SACOL_RS12530 ]
  • symbol: SACOL2388
  • description: MerR family transcriptional regulator
  • length: 246
  • theoretical pI: 7.48621
  • theoretical MW: 29126.9
  • GRAVY: -0.555285

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions Cu(I)-responsive transcriptional regulator (TIGR02044; HMM-score: 44.6)
    Signal transduction Regulatory functions DNA interactions Cd(II)/Pb(II)-responsive transcriptional regulator (TIGR02047; HMM-score: 40.5)
    Cellular processes Cellular processes Detoxification Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 39.6)
    Signal transduction Regulatory functions DNA interactions Hg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 39.6)
    Signal transduction Regulatory functions DNA interactions Zn(II)-responsive transcriptional regulator (TIGR02043; HMM-score: 36.7)
    and 2 more
    Cellular processes Cellular processes Detoxification redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 21.3)
    Signal transduction Regulatory functions DNA interactions redox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 21.3)
  • TheSEED  :
    • Transcriptional regulator, MerR family
  • PFAM:
    HTH (CL0123) MerR_1; MerR HTH family regulatory protein (PF13411; HMM-score: 60.7)
    no clan defined TipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 53.8)
    and 6 more
    HTH (CL0123) MerR; MerR family regulatory protein (PF00376; HMM-score: 42.3)
    MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 23.1)
    no clan defined MKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 14.6)
    FliD_N; Flagellar hook-associated protein 2 N-terminus (PF02465; HMM-score: 12.5)
    Ribosomal_L12_N; Ribosomal protein L7/L12 dimerisation domain (PF16320; HMM-score: 12.5)
    HTH (CL0123) MerR_2; MerR HTH family regulatory protein (PF13591; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0016
    • TAT(Tat/SPI): 0.00007
    • LIPO(Sec/SPII): 0.000362
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQQDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSVCEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTTIFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFNSYDNQNLASYISEAVNYFLSNVNKSDNF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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