PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2367 [new locus tag: SA_RS13560 ]
• pan locus tag^?: SAUPAN006257000
• symbol: SA2367
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2367 [new locus tag: SA_RS13560 ]
• symbol: SA2367
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2656966..2657796
• length: 831
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125296 NCBI
• RefSeq: NP_375693 NCBI
• BioCyc: see SA_RS13560
• MicrobesOnline: 104719 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGAAACTTTAGAATTACAAGGCGCTAAATTACGATACCACCAAGTCGGACAAGGACCC
  GTGCTCATCTTTATTCCTGGTGCAAACGGTACCGGAAACATTTTTCTGCCTCTTGCAGAA
  CAGTTAAAAGACCATTTTACTGTTGTAGCCGTTGATCGTCGTGATTATGGAGAAAGCGAG
  TTAACTGAACCACTCCCTGATTCCGCTTCAAACCCTGACAGTGATTATCGTGTCAAACGC
  GACGCACAGGACATTGCCGAACTTGCAAAGTCATTAAGTGATGAACCTGTCTATATATTA
  GGTTCAAGTTCAGGTTCAATCGTTGCGATGCATGTGTTAAAAGATTACCCTGAAGTCGTT
  AAAAAGATTGCATTTCACGAACCACCAATTAATACATTTTTACCAGACAGTACTTATTGG
  AAAGATAAAAATGATGACATTGTGCATCAAATTTTAACAGAAGGCTTAGAAAAAGGTATG
  AAAACATTTGGTGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCT
  GCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTT
  GAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGAT
  AAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTT
  TATATAAACAAAGAAACTGGCATACCTATTGTAGATATTCCAGGCGGTCACTTAGGTTAT
  ATTCAAAAACCTGAAGGCTTTGCTGATGTATTATTAAATATGTGGGGTTAA
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  831
  

⊟Protein

⊟General

• locus tag: SA2367 [new locus tag: SA_RS13560 ]
• symbol: SA2367
• description: hypothetical protein
• length: 276
• theoretical pI: 4.47643
• theoretical MW: 31004.7
• GRAVY: -0.426812

⊟Function

• reaction:
  EC 3.-.-.-?  ExPASy
• TIGRFAM:
  Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 41)
  and 7 more
  pyrimidine utilization protein D (TIGR03611; HMM-score: 31.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 30.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 28.1)
  proline-specific peptidase (TIGR01250; HMM-score: 24.8)
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 22.8)
  Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 18.3)
  prolyl aminopeptidase (TIGR01249; EC 3.4.11.5; HMM-score: 12.7)
• TheSEED  :
  • Predicted hydrolase/acyltransferase
• PFAM:
  AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 58.1)
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 47.3)
  and 3 more
  Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 31.9)
  Thioesterase; Thioesterase domain (PF00975; HMM-score: 15)
  Esterase; Putative esterase (PF00756; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010635
  • TAT(Tat/SPI): 0.001217
  • LIPO(Sec/SPII): 0.003426
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928160 NCBI
• RefSeq: NP_375693 NCBI
• UniProt: Q7A3C4 UniProt

⊟Protein sequence

• METLELQGAKLRYHQVGQGPVLIFIPGANGTGNIFLPLAEQLKDHFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGSSSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGMKTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKYSDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2364 > SA2365 > SA2366 > SA2367

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)