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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2597 [new locus tag: SACOL_RS13595 ]
  • pan locus tag?: SAUPAN006257000
  • symbol: SACOL2597
  • pan gene symbol?: —
  • synonym:
  • product: alpha/beta fold family hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2597 [new locus tag: SACOL_RS13595 ]
  • symbol: SACOL2597
  • product: alpha/beta fold family hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 2657439..2658269
  • length: 831
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGAAACTTTAGAATTACAAGGCGCTAAATTACGATACCACCAAGTCGGACAAGGACCC
    GTGCTCATCTTTATTCCTGGTGCAAACGGTACCGGAGACATTTTTCTGCCTCTTGCAGAA
    CAGTTAAAAGACCATTTTACTGTTGTAGCCGTTGATCGTCGTGATTATGGAGAAAGCGAG
    TTAACTGAACCACTCCCTGATTCCGCTTCAAACCCTGACAGTGATTATCGTGTCAAACGC
    GACGCACAGGACATTGCCGAACTTGCAAAGTCATTAAGTGATGAACCTGTCTATATATTA
    GGTTCAAGTTCAGGTTCAATCGTTGCGATGCATGTGTTAAAAGATTACCCTGAAGTCGTT
    AAAAAGATTGCATTTCACGAACCACCAATTAATACATTTTTACCAGACAGTACTTATTGG
    AAAGATAAAAATGATGACATTGTGCATCAAATTTTAACAGAAGGCTTAGAAAAAGGCATG
    AAAACATTTGGCGAAACATTAAATATCGCACCAATTGATGCAAAAATGATGTCTCAACCT
    GCTGATACTGAAGAAGGACGTATAGAACAGTACAAACGTACGATGTTCTGGTCAGAATTT
    GAAATTAGACAATATACGCATTCAGATATTACTTTGGATGATTTCACAAAATACAGCGAT
    AAAATCACCCTACTAAATGGGACAGATTCTAGAGGCTCCTTCCCACAAGATGTTAACTTT
    TATATAAATAAAGAAACTGGCATACCTATTGTAGATATTCCAGGCGGTCACTTAGGTTAT
    ATTCAAAAACCTGAAGGCTTTGCTGATGTATTATTAAATATGTGGGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    831

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2597 [new locus tag: SACOL_RS13595 ]
  • symbol: SACOL2597
  • description: alpha/beta fold family hydrolase
  • length: 276
  • theoretical pI: 4.43681
  • theoretical MW: 31005.7
  • GRAVY: -0.426812

⊟Function[edit | edit source]

  • reaction:
    EC 3.-.-.-?  ExPASy
  • TIGRFAM:
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 40.6)
    and 7 more
    pyrimidine utilization protein D (TIGR03611; HMM-score: 30.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 29.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 27.9)
    proline-specific peptidase (TIGR01250; HMM-score: 24.2)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 22.7)
    Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 18.4)
    prolyl aminopeptidase (TIGR01249; EC 3.4.11.5; HMM-score: 12.1)
  • TheSEED  :
    • Predicted hydrolase/acyltransferase
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 58.8)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 47.5)
    and 3 more
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 31.2)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 15.1)
    Esterase; Putative esterase (PF00756; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009449
    • TAT(Tat/SPI): 0.000963
    • LIPO(Sec/SPII): 0.003392
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKDHFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGSSSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGMKTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKYSDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 1595 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [5] [6]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 184.03 h [7]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  6. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)
  7. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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