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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2593 [new locus tag: SACOL_RS13580 ]
  • pan locus tag?: SAUPAN006252000
  • symbol: SACOL2593
  • pan gene symbol?: gbaA
  • synonym:
  • product: TetR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2593 [new locus tag: SACOL_RS13580 ]
  • symbol: SACOL2593
  • product: TetR family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 2655057..2655614
  • length: 558
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCGAAAAGATGCAAAAGAGAATAGGCAACGAATTGAAGAAATAGCGCACAAACTTTTT
    GATGAAGAAGGCGTTGAAAACATTAGTATGAATCGTATTGCTAAAGAACTAGGTATCGGT
    ATGGGGACATTGTATCGACATTTCAAAGATAAAAGCGATTTATGCTACTACGTCATACAA
    AGAGACCTTGATATTTTTATCACTCATTTTAAGCAAATTAAAGATGACTATCATTCTAAT
    TATGAGGTTATGCAAGTGTCGTTAGATTATCTTTTACAATTTAAAATCGATAATAAGGCA
    CTACTGCAATGTATTGAAGCAGGCAACAATAAATTACGTTTTTACCAAAGCGCATTTTAT
    CAAGAATTATTTGATTTTTATTATGACTTATTTAAAAGCGATGACGACACTTACACGAAG
    TTCAAAACAGATATGCTGTTGCAATCATTATCAACTAGTGTATTTGCCTTCCAAATAGAG
    CATCGCCATATTTCAATTGAAGCGTACAGAAATTATTTATTAAACATTTATTTAGATGAG
    GTGGGACGTAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    558

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2593 [new locus tag: SACOL_RS13580 ]
  • symbol: SACOL2593
  • description: TetR family transcriptional regulator
  • length: 185
  • theoretical pI: 5.33099
  • theoretical MW: 22260
  • GRAVY: -0.54

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 41.8)
    and 5 more
    pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 20.7)
    probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 20.6)
    Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 16)
    probable regulatory domain (TIGR03879; HMM-score: 13.5)
    Genetic information processing Mobile and extrachromosomal element functions Other probable addiction module antidote protein (TIGR02684; HMM-score: 12.6)
  • TheSEED  :
    • Transcriptional regulator, TetR family
  • PFAM:
    HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 46.6)
    and 7 more
    CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 16.2)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 14.6)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.7)
    no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 12.7)
    NPIP; Nuclear pore complex interacting protein (NPIP) (PF06409; HMM-score: 12.3)
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.1)
    4H_Cytokine (CL0053) IL12; Interleukin-12 alpha subunit (PF03039; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007128
    • TAT(Tat/SPI): 0.000387
    • LIPO(Sec/SPII): 0.001252
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTLYRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALLQCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIEHRHISIEAYRNYLLNIYLDEVGRND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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