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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0189 [new locus tag: SACOL_RS00965 ]
- pan locus tag?: SAUPAN001061000
- symbol: SACOL0189
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Additional information (user-provided)[edit | edit source]
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0189 [new locus tag: SACOL_RS00965 ]
- symbol: SACOL0189
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 222645..223418
- length: 774
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236414 NCBI
- RefSeq: YP_185088 NCBI
- BioCyc: see SACOL_RS00965
- MicrobesOnline: 911666 MicrobesOnline
⊟Phenotype[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟DNA sequence[edit | edit source]
- 1
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721ATGAAAGCACATAAAATTTTCTGGTTAAACTTAGCAGCAATTATTATTATTTCAATTGTA
GTCAGTGGCGATATGTTTTTGGCAATGAAGTGGGAACAAATTCATTTGAAAGATGGTCTT
AAAAAAGTACTAAGTACGTATCCTATTAAAAACTTAGAAACGCTTTATGAAATTGATGGT
CACGATAATCCCCATTATGAAAATAATGATCAAGACACATGGTATATAGAATCTTCTTAT
TCAGTTGTTGGATCAGATGAGCTTTTAAAAGAAGACCGTATGTTGTTAAAGGTAGATAAG
AACACACATAAAATAACTGGTGAATATGACACAACTACAAACGATAAAAAAAATGCTACT
GACTCGACATACAAAAGTTATCCAGTAAAAGTAGTTAATAATAAAATCGTTTTCACAAAA
GACGTAAAAGATCCCGCGCTGAAACAAAAAATCGAAAACAATCAATTTTTGATTCAAAGT
GGTGATTTAACAAGCATTTTAAATAGCAATGATTTGAAAGTCACACATGATCCTACCACT
GATTATTATAATTTATCTGGTAAGTTGTCGAACGATAATCCAAACGTTAAACAATTAAAA
CGTAGATATAATATTCCTAAAAACGCATCAACAAAGGTGGAATTAAAGGGAATGAGTGAT
TTAAAAGGCAATAATCATCAAGATCAGAAACTTTATTTTTATTTTTCAAGTCCTGGAAAA
GACCAAATCATTTATAAAGAAAGCCTTACTTATAATAAAATAAGTGAACATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0189 [new locus tag: SACOL_RS00965 ]
- symbol: SACOL0189
- description: hypothetical protein
- length: 257
- theoretical pI: 8.55475
- theoretical MW: 29648.3
- GRAVY: -0.729183
⊟Function[edit | edit source]
- TIGRFAM: Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 397.4)
- TheSEED :
- FIG01107907: hypothetical protein
- PFAM: LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 289.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helix: 1
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.9389
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0609
- LocateP: N-terminally anchored (No CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: 4
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide SP(Sec/SPI) length 28 aa
- SP(Sec/SPI): 0.465262
- TAT(Tat/SPI): 0.001453
- LIPO(Sec/SPII): 0.129551
- Cleavage Site: CS pos: 28-29. FLA-MK. Pr: 0.2087
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVLSTYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNTHKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQSGDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGMSDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Signal peptide containing [1] [2] [3]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Additional information (user-provided)[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Other information (user-provided)[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)