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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0479 [new locus tag: SACOL_RS02425 ]
  • pan locus tag?: SAUPAN002130000
  • symbol: SACOL0479
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0479 [new locus tag: SACOL_RS02425 ]
  • symbol: SACOL0479
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 480891..482399
  • length: 1509
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGTGAAAATTTTAAGTTACGTAAAATGAAAGTCGGTTTAGTATCTGTTGCAATTACA
    ATGTTATATATTATGACAAACGGACAAGCAGAAGCATCTGAAAATCAAAACGCTTTAATC
    TCTAATATAAATGTAGACAATCAGGAAAAACAGAATAATGTAAATCAAGCTGTTCAGCCT
    CAAAATAATACTAATGAAACATCAAAAGTACCGGCTAATTTTGTCAAATTGAATGATATT
    AAACCAGGTGATACTTCTATACAAGGAACAACTTTACCAAATCAATTTATACTATTAACT
    ATTGATAAAAAAGATGTGAGCTCAGTTGAAGATTCTGACAGCAGCTTTGTTATGTCTGAT
    AAAGATGGGAATTTTAAGTATGACTTAAATGGTCGCAAAATTGTTCATAATCAAGAAATT
    GAAGTGTCTTCATCAGATCCCTATTTAGGTGACGATGAAGAAGATGAAGAAGTAGAAGAA
    ACTTCAACTGAAGAAGTTGGTGCTGAGGAAGAAAGTACAGAAGCTAAAGCTACATATACA
    ACACCGCGATATGAAAAAGCGTATGAAATACCGAAAGAACAGCTAAAAGAAAAAGATGGA
    CATCACCAAGTTTTTATCGAACCTATTACTGAAGGTTCAGGTATTATTAAAGGCCATACC
    TCTGTAAAAGGTAAAGTTGCTCTATCTATTAATAATAAATTTATTAACTTTGAGACAAAT
    GCTAATGGTGGTCCAAATAAAGAAGAAGCGAAATCTGGATCAGAAGGAATCTGGATGCCT
    ATTGATGACAAAGGATACTTTAATTTTGACTTCAAAACGAAACGTTTCGATGATTTAGAG
    TTAAAGAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTG
    AAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACT
    AAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTA
    CATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGAT
    AAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAATGCAGTTGTGAAGGTA
    GACTCTGAACTAGGTGAAGGTCAAGAATTCCCTGATTTGCAAGTCGATGAAAAAGGTGAA
    TTCAGCTTTGATGTAGATCATGCTGGATTTAGATTACAAAATGGAGAAACACTAAACTTC
    ACAGTAGTTGATCCTATTACAGGTGAATTATTAAGTGGAAATTTTGTTTCTAAAAACATA
    GATATTTATGAATCTCCTGAAGAAAAAGCAGATCGTGAGTTTGATGAAAGAATGGAAAAC
    ACACCTGCATATCATAAATTACATGGTGATAAAATTGTCGGCTACGATACTAACGGATTC
    CCGATTACCTGGTTTTATCCATTAGGTGAAAAGAAAGTTGAACGTAAGGCACCAAAATTA
    GAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0479 [new locus tag: SACOL_RS02425 ]
  • symbol: SACOL0479
  • description: hypothetical protein
  • length: 502
  • theoretical pI: 4.4924
  • theoretical MW: 56477.3
  • GRAVY: -0.78506

⊟Function[edit | edit source]

  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 18.2)
  • TheSEED  :
    • Hypothetical protein within Pathogenicity island SaPIn2
  • PFAM:
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 15.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: GQAEASEN
  • SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.991876
    • TAT(Tat/SPI): 0.002411
    • LIPO(Sec/SPII): 0.001807
    • Cleavage Site: CS pos: 32-33. AEA-SE. Pr: 0.9484
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNINVDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTIDKKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEETSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRFDDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPDLQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKADREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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