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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0859 [new locus tag: SACOL_RS04410 ]
  • pan locus tag?: SAUPAN002797000
  • symbol: SACOL0859
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0859 [new locus tag: SACOL_RS04410 ]
  • symbol: SACOL0859
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 886899..887369
  • length: 471
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAAAGGAAAGTATTAGTACTAACAATGGGTGTAATTTGTGCAACTCAATTATGGCAT
    TCTAATCACGCAAACGCATTAGTAACAGAGAGTGGCGCAAATGATACTAAGCAATTTACT
    GAAATTGTATCGGAAGAGAAAGTTATAACAGTTGAACATGCTCAAATTAATATTTTTCAA
    TCTAATAGCAATTCAAACTTGATGGAGTTCAACATATTAACAATGGGCGGTAAATCAGGA
    GCTATGGTTGGTTATAGTGAAATTGACTCATCACATTTCACAGACCGTGACAAACGCGTT
    ATTAGACGTGATCATGTTAAAGAAGCACAAAGCTTAGTAGAGAACTATAAAGATACACAA
    AGTGCTGATGCTAGGATGAAAGCCAAACAAAAAGTTAACACATTAAGCAAACCGCATCAA
    AACTATTTCAATAAACAAATTGATAAGGTTTATAATGGATTACAACGCTAA
    60
    120
    180
    240
    300
    360
    420
    471

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0859 [new locus tag: SACOL_RS04410 ]
  • symbol: SACOL0859
  • description: hypothetical protein
  • length: 156
  • theoretical pI: 9.68999
  • theoretical MW: 17716.9
  • GRAVY: -0.628205

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Hypothetical protein, similarity with von Willebrand factor-binding VWbp
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Hypothetical protein, similarity with von Willebrand factor-binding VWbp
  • PFAM:
    no clan defined efb-c; Extracellular fibrinogen binding protein C terminal (PF12199; HMM-score: 15)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 4.6
    • Cellwall Score: 2.48
    • Extracellular Score: 2.92
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.989266
    • TAT(Tat/SPI): 0.002182
    • LIPO(Sec/SPII): 0.002696
    • Cleavage Site: CS pos: 26-27. ANA-LV. Pr: 0.9185
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVSEEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIRRDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]