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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0908 [new locus tag: SACOL_RS04660 ]
  • pan locus tag?: SAUPAN002880000
  • symbol: SACOL0908
  • pan gene symbol?: ear
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0908 [new locus tag: SACOL_RS04660 ]
  • symbol: SACOL0908
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 917356..917913
  • length: 558
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATAAAAAACTATTAACAAGAACATTGATAGCAAGTGCTTTAGTTTTAACAACAGTA
    GGTTCAGGTTTTCATTCTTCTTCAAATTATAATGGTATTAATAACGTTGCAAAGGCTTCT
    GAAATAACAGATAGAGACTTATGGAAAAATGTAAGAGATGCTTTGAAAGAAGCAAATATT
    ATCGATAAAACAGCAAATGAGACAGTTGGTGTTACGTACAATTTAAATAATGGTGGCGAA
    AGTAGTATTACGGGTACAGCTGATTTAGATGAACTTAGTAATTTTAATAACAAGCCGATT
    AATACCGATAGTGTTAAAAGAATTGATTTGTCAAGAATAAATCCAAATGGAAATAGGTTT
    GACGCAAATGATGCATGGAAAAAATTAACTGACAAATTAAAAGAGAAACAAATCGTTAAA
    AACGGCGACACAGTAACTATCCATAGTAAAGATAAAAATGATCCCCCAATTTCAGCTAAA
    GTTGGTGAAAACTATAATGGCAACAAGGGGTTAATGCTAAATCAGAGAGATATAACAAAA
    ATAACTATAACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    558

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0908 [new locus tag: SACOL_RS04660 ]
  • symbol: SACOL0908
  • description: hypothetical protein
  • length: 185
  • theoretical pI: 9.99344
  • theoretical MW: 20345.7
  • GRAVY: -0.692432

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 17.9)
  • TheSEED  :
    • Secreted protein Ear, superantigen-encoding pathogenicity islands SaPI
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Secreted protein Ear, superantigen-encoding pathogenicity islands SaPI
  • PFAM:
    no clan defined DUF4888; Domain of unknown function (DUF4888) (PF16229; HMM-score: 75.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 39 aa
    • SP(Sec/SPI): 0.757326
    • TAT(Tat/SPI): 0.010019
    • LIPO(Sec/SPII): 0.181312
    • Cleavage Site: CS pos: 39-40. AKA-SE. Pr: 0.4390
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKKLLTRTLIASALVLTTVGSGFHSSSNYNGINNVAKASEITDRDLWKNVRDALKEANIIDKTANETVGVTYNLNNGGESSITGTADLDELSNFNNKPINTDSVKRIDLSRINPNGNRFDANDAWKKLTDKLKEKQIVKNGDTVTIHSKDKNDPPISAKVGENYNGNKGLMLNQRDITKITITK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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