PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0815 [new locus tag: SAUSA300_RS04395 ]
• pan locus tag^?: SAUPAN002880000
• symbol: ear
• pan gene symbol^?: ear
• synonym:
• product: Ear protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0815 [new locus tag: SAUSA300_RS04395 ]
• symbol: ear
• product: Ear protein
• replicon: chromosome
• strand: -
• coordinates: 894575..895129
• length: 555
• essential: unknown

⊟Accession numbers

• Gene ID: 3914463 NCBI
• RefSeq: YP_493515 NCBI
• BioCyc: see SAUSA300_RS04395
• MicrobesOnline: 1292330 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATAAAAAACTATTAACAAAAACATTGATAGCAAGTGCTTTAGTTTTAACAACAGTA
  GGTTCAGGTTTTCATTCTTCTTCAAATTATAATGGTATTAATAACGTTGCAAAAGCTGAG
  CAAACGACAGATAACGAATTGTGGAAAAATGTAAGAGACGCTTTAAAAGAAGCGAATATT
  ATCGATAAAACAGATAATGAAAATGTCAAAGTGACTTATAAATTAGCAAATGGCGGTGAA
  AACAGTATTGAAGGTACCGCCAATTTAAATGAACTTAGCAATTCTAATAACAAACCGATC
  AATAGCGATAGTGTAAAAAGCATTGATTTGTCAAGGAAAAATCCCAATGGAGATAGATTT
  GACGCAAATGATGCATGGAAAAAATTAACTGATAAATTAAAAGAAAAGCAGATTGTGAAA
  GACGGTGATACAGTAACTATTCATAGTAAAGACGAAAAAGACCCTAAAATCTCAGCTAAA
  GTCGGTGAAGTCTATAATGATAACAAACGATTGATGTTGTTTAAAAAGGATATAGATAAA
  ATCACTATAAACTAG
  60
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  555
  

⊟Protein

⊟General

• locus tag: SAUSA300_0815 [new locus tag: SAUSA300_RS04395 ]
• symbol: Ear
• description: Ear protein
• length: 184
• theoretical pI: 9.56215
• theoretical MW: 20436.8
• GRAVY: -0.826087

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 19.8)
• TheSEED  :
  • Secreted protein Ear, superantigen-encoding pathogenicity islands SaPI
  Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Secreted protein Ear, superantigen-encoding pathogenicity islands SaPI
• PFAM:
  no clan defined DUF4888; Domain of unknown function (DUF4888) (PF16229; HMM-score: 69.1)
  and 2 more
  Terminase_4; Phage terminase, small subunit (PF05119; HMM-score: 15.1)
  PK_C; Pyruvate kinase, alpha/beta domain (PF02887; HMM-score: 14.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 2
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 39 aa
  • SP(Sec/SPI): 0.778921
  • TAT(Tat/SPI): 0.003446
  • LIPO(Sec/SPII): 0.188177
  • Cleavage Site: CS pos: 39-40. AKA-EQ. Pr: 0.4141
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161698 NCBI
• RefSeq: YP_493515 NCBI
• UniProt: A0A0H2XJB4 UniProt

⊟Protein sequence

• MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTDNELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINSDSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAKVGEVYNDNKRLMLFKKDIDKITIN

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications