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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04395 [old locus tag: SAUSA300_0815 ]
  • pan locus tag?: SAUPAN002880000
  • symbol: SAUSA300_RS04395
  • pan gene symbol?: ear
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04395 [old locus tag: SAUSA300_0815 ]
  • symbol: SAUSA300_RS04395
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 894575..895129
  • length: 555
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATAAAAAACTATTAACAAAAACATTGATAGCAAGTGCTTTAGTTTTAACAACAGTA
    GGTTCAGGTTTTCATTCTTCTTCAAATTATAATGGTATTAATAACGTTGCAAAAGCTGAG
    CAAACGACAGATAACGAATTGTGGAAAAATGTAAGAGACGCTTTAAAAGAAGCGAATATT
    ATCGATAAAACAGATAATGAAAATGTCAAAGTGACTTATAAATTAGCAAATGGCGGTGAA
    AACAGTATTGAAGGTACCGCCAATTTAAATGAACTTAGCAATTCTAATAACAAACCGATC
    AATAGCGATAGTGTAAAAAGCATTGATTTGTCAAGGAAAAATCCCAATGGAGATAGATTT
    GACGCAAATGATGCATGGAAAAAATTAACTGATAAATTAAAAGAAAAGCAGATTGTGAAA
    GACGGTGATACAGTAACTATTCATAGTAAAGACGAAAAAGACCCTAAAATCTCAGCTAAA
    GTCGGTGAAGTCTATAATGATAACAAACGATTGATGTTGTTTAAAAAGGATATAGATAAA
    ATCACTATAAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    555

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS04395 [old locus tag: SAUSA300_0815 ]
  • symbol: SAUSA300_RS04395
  • description: hypothetical protein
  • length: 184
  • theoretical pI: 9.56215
  • theoretical MW: 20436.8
  • GRAVY: -0.826087

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 19.8)
  • TheSEED: see SAUSA300_0815
  • PFAM:
    no clan defined DUF4888; Domain of unknown function (DUF4888) (PF16229; HMM-score: 69.1)
    and 2 more
    Terminase_4; Phage terminase, small subunit (PF05119; HMM-score: 15.1)
    PK_C; Pyruvate kinase, alpha/beta domain (PF02887; HMM-score: 14.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 39 aa
    • SP(Sec/SPI): 0.778921
    • TAT(Tat/SPI): 0.003446
    • LIPO(Sec/SPII): 0.188177
    • Cleavage Site: CS pos: 39-40. AKA-EQ. Pr: 0.4141
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTDNELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINSDSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAKVGEVYNDNKRLMLFKKDIDKITIN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]