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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1251 [new locus tag: SACOL_RS06390 ]
- pan locus tag?: SAUPAN003522000
- symbol: SACOL1251
- pan gene symbol?: ftsY
- synonym:
- product: cell division protein FtsY
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1251 [new locus tag: SACOL_RS06390 ]
- symbol: SACOL1251
- product: cell division protein FtsY
- replicon: chromosome
- strand: +
- coordinates: 1260104..1261354
- length: 1251
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3238080 NCBI
- RefSeq: YP_186110 NCBI
- BioCyc: see SACOL_RS06390
- MicrobesOnline: 912716 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTT
AAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCA
ACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTG
AGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAA
GCTCAAAAAATGGGTGCTAAATTTAAAGCAGGACTCGAAAAATCTCGTCAAAATTTCCAA
GAACAATTAAATAATTTGATAGCGAGATATCGTAAAGTAGATGAAGACTTTTTTGAAGCT
TTAGAAGAAATGTTAATCACTGCAGACGTCGGTTTTAATACAGTGATGACGTTAACTGAA
GAATTACGTATGGAAGCACAACGACGTAATATTCAAGATACTGAAGATTTGCGTGAAGTC
ATTGTTGAAAAAATCGTAGAGATTTACCATCAAGAAGATGATAATTCAGAAGCTATGAAC
TTAGAAGATGGTCGTTTAAATGTCATTTTAATGGTTGGTGTGAATGGTGTTGGTAAAACA
ACAACAATTGGAAAATTAGCTTACCGATATAAAATGGAAGGTAAAAAAGTAATGTTAGCT
GCGGGCGATACTTTTAGAGCGGGTGCTATTGATCAATTGAAAGTTTGGGGCGAACGTGTT
GGTGTAGACGTAATTAGCCAAAGTGAAGGTTCTGATCCAGCTGCTGTTATGTATGATGCG
ATTAATGCCGCTAAAAACAAAGGTGTTGATATTTTAATCTGTGATACCGCTGGACGTTTA
CAAAATAAAACAAATCTAATGCAAGAATTAGAAAAAGTTAAGCGTGTAATTAATCGAGCA
GTGCCAGATGCGCCTCATGAAGCATTACTATGTTTAGATGCTACAACTGGTCAGAATGCG
TTGTCACAAGCTAGAAACTTTAAAGAAGTAACAAATGTTACAGGTATTGTATTAACGAAA
TTAGATGGTACAGCCAAAGGTGGTATCGTATTAGCCATTCGTAATGAATTGCACATCCCA
GTTAAATATGTAGGTTTAGGTGAGCAATTAGATGACTTACAACCATTTAACCCTGAAAGT
TATGTCTACGGCTTATTCGCTGATATGATTGAACAAAATGAAGAAATAACAACAGTTGAA
AATGATCAAATTGTAACAGAAGAAAAGGACGATAATCATGGGTCAAAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1251 [new locus tag: SACOL_RS06390 ]
- symbol: SACOL1251
- description: cell division protein FtsY
- length: 416
- theoretical pI: 4.39014
- theoretical MW: 46590
- GRAVY: -0.545673
⊟Function[edit | edit source]
- TIGRFAM: Protein fate Protein and peptide secretion and trafficking signal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 388.5)and 11 moreProtein fate Protein and peptide secretion and trafficking signal recognition particle protein (TIGR00959; HMM-score: 233.6)signal recognition particle protein SRP54 (TIGR01425; HMM-score: 185.8)Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 91.4)Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 18.9)Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 16)Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 15.7)Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 13.2)Unknown function General putative selenium-dependent hydroxylase accessory protein YqeC (TIGR03172; HMM-score: 12.9)thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 12)
- TheSEED :
- Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)and 1 more - PFAM: P-loop_NTPase (CL0023) SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 273.5)and 16 moreno clan defined SRP54_N; SRP54-type protein, helical bundle domain (PF02881; HMM-score: 48.9)P-loop_NTPase (CL0023) ArgK; ArgK protein (PF03308; HMM-score: 19)AAA_30; AAA domain (PF13604; HMM-score: 18.6)AAA_17; AAA domain (PF13207; HMM-score: 16.9)ABC_tran; ABC transporter (PF00005; HMM-score: 15.5)CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 15.2)AAA_31; AAA domain (PF13614; HMM-score: 15.2)AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.7)AAA_22; AAA domain (PF13401; HMM-score: 14.5)Zeta_toxin; Zeta toxin (PF06414; HMM-score: 14.3)AAA_33; AAA domain (PF13671; HMM-score: 14.1)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.4)AAA_24; AAA domain (PF13479; HMM-score: 13.2)APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 12.8)AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 9.5)no clan defined Scm3; Centromere protein Scm3 (PF10384; HMM-score: 6.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007941
- TAT(Tat/SPI): 0.001861
- LIPO(Sec/SPII): 0.0026
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDANDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQLNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREVIVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVMLAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDTAGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVTGIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQNEEITTVENDQIVTEEKDDNHGSK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3] [4]
- quantitative data / protein copy number per cell: 543 [5]
- interaction partners:
SACOL0452 (ahpC) alkyl hydroperoxide reductase subunit C [6] (data from MRSA252) SACOL0842 (eno) phosphopyruvate hydratase [6] (data from MRSA252) SACOL1329 (femC) glutamine synthetase [6] (data from MRSA252) SACOL1734 (gapA2) glyceraldehyde 3-phosphate dehydrogenase 2 [6] (data from MRSA252) SACOL1741 (icd) isocitrate dehydrogenase [6] (data from MRSA252) SACOL1274 (rpsB) 30S ribosomal protein S2 [6] (data from MRSA252) SACOL2233 (rpsC) 30S ribosomal protein S3 [6] (data from MRSA252) SACOL2222 (rpsE) 30S ribosomal protein S5 [6] (data from MRSA252) SACOL2214 (rpsK) 30S ribosomal protein S11 [6] (data from MRSA252) SACOL1449 (sucA) 2-oxoglutarate dehydrogenase E1 component [6] (data from MRSA252) SACOL0594 (tuf) elongation factor Tu [6] (data from MRSA252) SACOL2104 (upp) uracil phosphoribosyltransferase [6] (data from MRSA252) SACOL2553 pyruvate oxidase [6] (data from MRSA252) SACOL2561 hydroxymethylglutaryl-CoA synthase [6] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SACOL1250 > SACOL1251 > SACOL1252 > ffh
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: 65.44 h [7]
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
J Proteome Res: 2010, 9(3);1579-90
[PubMed:20108986] [WorldCat.org] [DOI] (I p) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)