From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1866 [new locus tag: SACOL_RS09570 ]
  • pan locus tag?: SAUPAN004559000
  • symbol: SACOL1866
  • pan gene symbol?: splD
  • synonym:
  • product: serine protease SplD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1866 [new locus tag: SACOL_RS09570 ]
  • symbol: SACOL1866
  • product: serine protease SplD
  • replicon: chromosome
  • strand: -
  • coordinates: 1918321..1919040
  • length: 720
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATATAATCATCAAAAGTATTGCGGCATTGACGATTTTAACATCAATAACT
    GGTGTCGGCACAACAGTGGTTGATGGTATTCAACAAACAGCCAAAGCAGAAAATAGTGTG
    AAATTAATTACCAACACGAATGTTGCACCATACAGTGGTGTTACATGGATGGGCGCTGGA
    ACAGGATTTGTAGTTGGGAATCAAACAATCATTACCAATAAACATGTTACTTATCACATG
    AAAGTCGGTGATGAAATCAAAGCACATCCTAATGGTTTTTATAATAACGGTGGTGGACTT
    TATAAAGTTACTAAGATTGTAGATTATCCTGGTAAAGAAGATATTGCGGTCGTACAAGTT
    GAAGAAAAATCAACGCAACCAAAAGGTAGAAAATTCAAAGATTTCACTAGCAAATTTAAT
    ATAGCATCAGAAGCTAAAGAAAATGAACCTATATCAGTCATTGGTTATCCAAATCCTAAT
    GGAAATAAACTACAAATGTATGAATCAACTGGTAAAGTACTATCAGTGAATGGAAATATA
    GTGACATCTGATGCGGTTGTCCAACCTGGCAGCTCTGGTTCACCTATATTAAATAGTAAG
    CGAGAAGCAATTGGTGTTATGTATGCTAGTGATAAACCAACAGGTGAAAGTACAAGGTCA
    TTTGCTGTTTATTTCTCTCCTGAAATTAAGAAATTTATTGCAGATAATTTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1866 [new locus tag: SACOL_RS09570 ]
  • symbol: SACOL1866
  • description: serine protease SplD
  • length: 239
  • theoretical pI: 9.67655
  • theoretical MW: 25669
  • GRAVY: -0.274059

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 27.5)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 27.5)
    and 1 more
    Genetic information processing Transcription Degradation of RNA inner membrane protein YhjD (TIGR00766; HMM-score: 10.3)
  • TheSEED  :
    • serine protease
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 64.6)
    Trypsin; Trypsin (PF00089; HMM-score: 55.3)
    and 4 more
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 26.4)
    Peptidase_S29; Hepatitis C virus NS3 protease (PF02907; HMM-score: 14.3)
    DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 13.7)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 8.8
    • Cellwall Score: 0.22
    • Extracellular Score: 0.98
    • Internal Helices: 2
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.922429
    • TAT(Tat/SPI): 0.00551
    • LIPO(Sec/SPII): 0.025381
    • Cleavage Site: CS pos: 36-37. AKA-EN. Pr: 0.7557
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLITNTNVAPYSGVTWMGAGTGFVVGNQTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGESTRSFAVYFSPEIKKFIADNLDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL1866&oldid=98169"