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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1864 [new locus tag: SACOL_RS09560 ]
  • pan locus tag?: SAUPAN004558000
  • symbol: SACOL1864
  • pan gene symbol?: splF
  • synonym:
  • product: serine protease SplF

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1864 [new locus tag: SACOL_RS09560 ]
  • symbol: SACOL1864
  • product: serine protease SplF
  • replicon: chromosome
  • strand: -
  • coordinates: 1916577..1917296
  • length: 720
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATATAATCATCAAAAGTATTGCAGCATTGACGATTTTAACATCAATAACT
    GGTGTCGGCACAACAATGGTTGAAGGTATTCAACAAACAGCCAAAGCCGAAAATACTGTT
    AAACAAATTACAAATACAAATGTTGCACCATACAGTGGTGTTACATGGATGGGCGCTGGA
    ACAGGATTTGTAGTTGGAAATCATACAATCATTACCAATAAACATGTTACCTATCACATG
    AAAGTCGGTGATGAAATCAAAGCACATCCTAATGGTTTTTATAATAACGGTGGTGGACTT
    TATAAAGTTACTAAGATTGTAGATTATCCTGGTAAAGAAGATATTGCGGTTGTACAAGTT
    GAAGAAAAATCAACACAACCAAAAGGTAGAAAATTCAAAGATTTCACTAGTAAATTTAAT
    ATAGCATCAGAAGCTAAAGAAAATGAACCTATATCAGTCATTGGTTATCCAAATCCTAAT
    GGAAATAAACTACAAATGTATGAATCAACTGGTAAAGTATTATCAGTGAATGGGAATATA
    GTGTCTTCGGATGCAATTATTCAGCCTGGTAGCTCTGGTTCACCTATATTAAATAGTAAA
    CACGAAGCTATTGGTGTAATCTATGCCGGTAATAAGCCATCAGGTGAAAGCACAAGAGGA
    TTTGCTGTTTATTTCTCTCCTGAAATTAAGAAATTCATTGCAGATAATTTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1864 [new locus tag: SACOL_RS09560 ]
  • symbol: SACOL1864
  • description: serine protease SplF
  • length: 239
  • theoretical pI: 9.6769
  • theoretical MW: 25655
  • GRAVY: -0.291213

Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.9)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.9)
  • TheSEED:  
    serine protease
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 62.5)
    Trypsin; Trypsin (PF00089; HMM-score: 58.9)
    and 3 more
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 24.2)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 16.9)
    Peptidase_S29; Hepatitis C virus NS3 protease (PF02907; HMM-score: 12.7)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.954299
    • TAT(Tat/SPI): 0.005688
    • LIPO(Sec/SPII): 0.014517
    • Cleavage Site: CS pos: 36-37. AKA-EN. Pr: 0.7724
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQITNTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]