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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1867 [new locus tag: SACOL_RS09575 ]
  • pan locus tag?: SAUPAN004564000
  • symbol: splC
  • pan gene symbol?: splC
  • synonym:
  • product: serine protease SplC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1867 [new locus tag: SACOL_RS09575 ]
  • symbol: splC
  • product: serine protease SplC
  • replicon: chromosome
  • strand: -
  • coordinates: 1919161..1919880
  • length: 720
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATATAGTCATTAAAAGCATGGCAGCATTAGCCATTCTAACCTCAGTAACT
    GGAATAAATGCTGCAGTCGTTGAAGAGACACAACAAATAGCAAATGCAGAGAAGAATGTT
    ACGCAAGTTAAAGATACAAATATTTTTCCATATAATGGCGTCGTTTCATTTAAAGATGCG
    ACAGGTTTTGTAATTGGAAAAAATACAATTATCACCAATAAACATGTATCAAAAGATTAT
    AAAGTTGGCGATAGAATTACTGCCCATCCAAACGGTGACAAAGGAAATGGTGGTATATAT
    AAAATTAAAAGCATTTCTGATTATCCGGGTGATGAAGACATCTCTGTCATGAATATTGAA
    GAACAAGCAGTCGAACGTGGACCAAAAGGCTTTAATTTTAATGAAAATGTCCAAGCATTC
    AATTTTGCGAAAGATGCTAAAGTTGATGACAAAATTAAAGTTATTGGTTACCCATTACCT
    GCTCAAAATAGTTTTAAACAGTTTGAATCTACAGGAACTATAAAAAGAATCAAAGACAAT
    ATTTTAAATTTTGATGCATACATTGAACCCGGGAATTCAGGATCACCAGTTCTAAATTCT
    AACAATGAGGTCATAGGTGTGGTGTATGGCGGTATTGGAAAAATTGGTTCTGAATATAAT
    GGTGCCGTATACTTTACGCCTCAAATCAAAGATTTTATTCAAAAGCACATTGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

⊟Protein[edit | edit source]

Protein Data Bank: 2AS9

⊟General[edit | edit source]

  • locus tag: SACOL1867 [new locus tag: SACOL_RS09575 ]
  • symbol: SplC
  • description: serine protease SplC
  • length: 239
  • theoretical pI: 6.81386
  • theoretical MW: 26098.2
  • GRAVY: -0.348117

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 18.1)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 18.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 15.2)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 15.2)
  • TheSEED  :
    • serine protease
  • PFAM:
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 85.7)
    and 6 more
    Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 53.1)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 19.9)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19.4)
    DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 14.6)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 13.4)
    no clan defined DUF3836; Family of unknown function (DUF3836) (PF12930; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: AVVEETQQ
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.980234
    • TAT(Tat/SPI): 0.004245
    • LIPO(Sec/SPII): 0.00665
    • Cleavage Site: CS pos: 36-37. ANA-EK. Pr: 0.5178
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVKDTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKIKSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLPAQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSEYNGAVYFTPQIKDFIQKHIEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Signal peptide containing [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

A K Ziebandt, H Weber, J Rudolph, R Schmid, D Höper, S Engelmann, M Hecker
Extracellular proteins of Staphylococcus aureus and the role of SarA and sigma B.
Proteomics: 2001, 1(4);480-93
[PubMed:11681202] [WorldCat.org] [DOI] (P p)

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