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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1869 [new locus tag: SACOL_RS09585 ]
- pan locus tag?: SAUPAN004567000
- symbol: splA
- pan gene symbol?: splA
- synonym:
- product: serine protease SplA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1869 [new locus tag: SACOL_RS09585 ]
- symbol: splA
- product: serine protease SplA
- replicon: chromosome
- strand: -
- coordinates: 1920785..1921501
- length: 717
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237977 NCBI
- RefSeq: YP_186697 NCBI
- BioCyc: see SACOL_RS09585
- MicrobesOnline: 913311 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGAATAAAAATGTAATGGTTAAAGGTTTAACTGCTTTAACTATTTTAACTATTTTAACA
TCTCTTGGTTTTGCTGAAAATATTTCTAATCAGCCTCATTCAATTGCCAAAGCAGAAAAG
AATGTCAAAGAAATTACCGATGCAACTAAGGAACCATACAATTCAGTGGTAGCATTTGTG
GGTGGTACTGGTGTAGTTGTTGGTAAAAATACAATCGTAACTAACAAACATATCGCTAAA
AGTAATGATATTTTTAAAAATAGAGTATCAGCACATCATTCGAGTAAAGGTAAAGGCGGA
GGAAACTACGACGTTAAAGACATTGTAGAATATCCCGGAAAAGAAGACCTTGCGATAGTT
CATGTTCATGAAACAAGTACAGAAGGTTTGAATTTTAATAAGAACGTTAGTTATACAAAA
TTTGCAGACGGAGCAAAAGTGAAAGATAGAATTTCTGTTATTGGTTATCCAAAGGGTGCA
CAAACAAAATATAAAATGTTTGAATCGACAGGAACGATTAACCATATCAGTGGAACGTTT
ATGGAATTTGATGCGTATGCACAACCAGGTAATTCAGGATCTCCTGTATTGAATTCTAAA
CATGAACTGATTGGTATTTTATATGCAGGTAGTGGAAAAGATGAATCTGAAAAGAATTTC
GGTGTTTATTTCACACCACAATTAAAAGAATTTATTCAAAATAATATTGAAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1869 [new locus tag: SACOL_RS09585 ]
- symbol: SplA
- description: serine protease SplA
- length: 238
- theoretical pI: 9.51102
- theoretical MW: 25876.1
- GRAVY: -0.393697
⊟Function[edit | edit source]
- reaction: EC 3.4.21.-? ExPASy
- TIGRFAM: Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 29)Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 29)Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.5)Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.5)
- TheSEED :
- serine protease
- PFAM: Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 73.8)Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 59.4)and 5 morePeptidase_S46; Peptidase S46 (PF10459; HMM-score: 24.5)Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 20.6)Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19.4)DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 17.3)Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 12.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.18
- Extracellular Score: 9.73
- Internal Helix: 1
- LocateP: N-terminally anchored (with CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: -0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: NISNQPHS
- SignalP: Signal peptide SP(Sec/SPI) length 38 aa
- SP(Sec/SPI): 0.956508
- TAT(Tat/SPI): 0.003891
- LIPO(Sec/SPII): 0.01994
- Cleavage Site: CS pos: 38-39. AKA-EK. Pr: 0.4480
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNKNVMVKGLTALTILTILTSLGFAENISNQPHSIAKAEKNVKEITDATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTKYKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFGVYFTPQLKEFIQNNIEK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: SaeR (activation) regulon
SaeR (TF) important in Virulence; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
A K Ziebandt, H Weber, J Rudolph, R Schmid, D Höper, S Engelmann, M Hecker
Extracellular proteins of Staphylococcus aureus and the role of SarA and sigma B.
Proteomics: 2001, 1(4);480-93
[PubMed:11681202] [WorldCat.org] [DOI] (P p)