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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2164 [new locus tag: SACOL_RS11375 ]
  • pan locus tag?: SAUPAN005564000
  • symbol: SACOL2164
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2164 [new locus tag: SACOL_RS11375 ]
  • symbol: SACOL2164
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2242249..2243619
  • length: 1371
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGATAGCTTTTCAAGGAAAGAGATAGTTATTGGGAGACTTAAGTTTATTACGATGTCT
    TTAATTGGTATCTTATTATTTTTAGTACCAATTCCAGTCGAACAAGATGGACAGAAGCAA
    ACAACGTTGCCCGTGGCATTTTTAGCAGGCGTGCTTAAAGATGTATTAGGTGGCGTAATG
    CCATTTTTAATTGTGACGATAATTACTTTATCGGGTATCATAACTCTGATTTGTTCAACT
    ATTTTAAAAGATAAATTGAAACCTGACGGGCTAATGAACAATGCTTTTAACGTTAGAATT
    GGTTGGTTAATTTTACGAATATTAGCAGTTGTTTTTGCATGGATGACATTTTTACGAATT
    GGTTCAAAGGTTATTTACTCAGATGAAACGGGTGGATTATTGTTCTCAAGTTTATTGCCA
    ACATTAGTGGCAGTATTCTTATTTGCAGCTCTATTTTTACCGCTATTAATGGAATACGGT
    TTGTTAGAGATGTTGGGTCCTATTTTTAGACCGGTTATGAGACCTTTATTTACATTGCCA
    GGACGTTCGACGGTAGATAATTTAGCATCATTTATTGGAGACGGAACTGTTGGTGTCTTA
    ATTACAAGTAGACAATATGGTGAAGGATATTATTCTCGTCGTGAGGCAACAGTTATTTCA
    ACGACATTTAGTGTAGTTTCTATCACCTTTGCAATTGTAGTTGCTGAAACAGTGCACATG
    CAAAATCAATTTTTTGCTTTCTATTTATCAGTGATAGTATCCTGCCTCGTTGCAGCAGTC
    ATTATGCCAAGAATTTGGCCTTTAAATAAAATTCCTGATGAGTATGCTAAGGAGGTACCT
    GAAAGTGCGCGTACTGAGGCATTGCCAGAAGGAAAGACGGCATTGAGACATGGCTTTGAT
    ACTGCAACAGAAGTAGGTATTAAAGCACCGGGCGTTATTGACTTTTTTAAGTCAGGATTA
    AAAACAGTCATTGATATGTGGTTTGTTATTTTGCCTGTAGTAATGAGTATTGGTACTATT
    GCTACCATTATTGCGAACTACACGCCCTTTTTTGTCATATTGGGTAAACCTTTTGTTCCA
    TTTTTAGAGTTAATGCAAATACCTGAAGCGGCACAGGCTTCTCAAACGATTATTATCGGT
    TTTGCCGATATGTTTTTACCTTCTATATTAATTGAAGGTGTACAAAATGATATAACTCGT
    TTTGTTATTGGGGCACTTAGTATATCGCAGTTAATATACTTGTCTGAAGTAGGTGGCGTC
    ATACTTGGTTCGAAGATTCCTGTAAGCATCGGCAAATTGTTTATGATTTTCTTGATTAGA
    ACAATCATTACACTACCAATTATTTCTTTGATGGCACATCTTTTGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL2164 [new locus tag: SACOL_RS11375 ]
  • symbol: SACOL2164
  • description: hypothetical protein
  • length: 456
  • theoretical pI: 8.17607
  • theoretical MW: 50005.7
  • GRAVY: 0.860526

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination sporulation integral membrane protein YlbJ (TIGR02871; HMM-score: 13.4)
  • TheSEED  :
    • Predicted arginine uptake transporter
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Predicted arginine uptake transporter
  • PFAM:
    no clan defined Gate; Nucleoside recognition (PF07670; HMM-score: 31.9)
    and 1 more
    T_cell_tran_alt; T-cell leukemia translocation-altered (PF15128; HMM-score: 16.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.131784
    • TAT(Tat/SPI): 0.007713
    • LIPO(Sec/SPII): 0.01097
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLPVAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGWLILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYGLLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATVISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYAKEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVVMSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIEGVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISLMAHLLL

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]