From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2384 [new locus tag: SACOL_RS12510 ]
  • pan locus tag?: SAUPAN005913000
  • symbol: sarZ
  • pan gene symbol?: sarZ
  • synonym:
  • product: accessory protein Z

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2384 [new locus tag: SACOL_RS12510 ]
  • symbol: sarZ
  • product: accessory protein Z
  • replicon: chromosome
  • strand: +
  • coordinates: 2443605..2444051
  • length: 447
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGTATGTAGAAAACAGCTATCTTAGCAAACAGTTGTGTTTTTTATTTTATGTTTCTTCA
    AAAGAAATAATTAAAAAATACACAAACTATCTTAAGGAATATGATTTAACCTATACTGGT
    TACATTGTTTTAATGGCGATTGAAAATGATGAAAAACTTAACATCAAAAAATTAGGTGAA
    CGTGTGTTCTTAGATTCTGGAACACTGACACCATTACTAAAGAAATTAGAAAAGAAAGAT
    TACGTTGTTCGAACACGTGAAGAGAAAGATGAAAGAAACCTACAAATTTCTCTAACAGAA
    CAAGGTAAAGCAATAAAAAGCCCTCTTGCTGAAATTTCTGTTAAGGTCTTTAATGAATTC
    AACATTTCAGAACGAGAAGCATCAGATATAATTAATAATTTGCGGAATTTTGTTTCTAAA
    AATTTTGACTATTCCGACAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    447

⊟Protein[edit | edit source]

Protein Data Bank: 3HRM
Protein Data Bank: 3HSE
Protein Data Bank: 3HSR
Protein Data Bank: 4GXO

⊟General[edit | edit source]

  • locus tag: SACOL2384 [new locus tag: SACOL_RS12510 ]
  • symbol: SarZ
  • description: accessory protein Z
  • length: 148
  • theoretical pI: 8.82125
  • theoretical MW: 17434.9
  • GRAVY: -0.526351

⊟Function[edit | edit source]

  • TIGRFAM:
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 46.8)
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 43.7)
    and 2 more
    mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 30.8)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, Acidobacterial, PadR-family (TIGR03433; HMM-score: 14.4)
  • TheSEED  :
    • Transcriptional regulator SarZ (Staphylococcal accessory regulator Z)
  • PFAM:
    HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 47.8)
    and 8 more
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 36.9)
    MarR_2; MarR family (PF12802; HMM-score: 28.2)
    HTH_34; Winged helix DNA-binding domain (PF13601; HMM-score: 23.2)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19)
    Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 17.7)
    PadR; Transcriptional regulator PadR-like family (PF03551; HMM-score: 17.5)
    HxlR; HxlR-like helix-turn-helix (PF01638; HMM-score: 16.8)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006838
    • TAT(Tat/SPI): 0.000141
    • LIPO(Sec/SPII): 0.012834
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVLMAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQGKAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 127 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 8.38 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Stephan Fuchs, Jan Pané-Farré, Christian Kohler, Michael Hecker, Susanne Engelmann
Anaerobic gene expression in Staphylococcus aureus.
J Bacteriol: 2007, 189(11);4275-89
[PubMed:17384184] [WorldCat.org] [DOI] (P p)

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2384&oldid=97973"