From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2533 [new locus tag: SACOL_RS13270 ]
  • pan locus tag?: SAUPAN006167000
  • symbol: SACOL2533
  • pan gene symbol?: —
  • synonym:
  • product: glyoxalase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2533 [new locus tag: SACOL_RS13270 ]
  • symbol: SACOL2533
  • product: glyoxalase
  • replicon: chromosome
  • strand: +
  • coordinates: 2594247..2595053
  • length: 807
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCATTTCACGATAAAACAGCAACACAAGTGACAAACATTGTACTGAACGTAAGAGAC
    TTAGACTTAATGACAACATTTTACAAAAACATTTTAGGACTATCCGTTAAAAGTTCTGAC
    GATAATACAACCGTATTATCTGTTGGGACTGGTGGCCATACTCTAACGTTACATTTATTA
    GAAGACGGCCGTCAGACTTCCCCACGTGAAGCAGGGCTTTTTCATATAGCATTTTTATTA
    CCAACTACTGAGGATCTAGCTAACTTCTTATATTTCGTGGCACAAAAAAATATGGGCATC
    GGCGCTGGTGATCATTTAGTAAGTGAAGCTTTATATTTCAACGACCCCGAAGGTAATGGT
    ATTGAAGTCTATCGCGATAGACCTTCATCTTCATGGGAATGGCAAAATGGCAAAGTTAAA
    ATGGATACTTTAGAAGTTGATAGCCAAACCTTATTAACACATCGTACTGATGAAGGTTGG
    CAAGGAATGCCAGCAAAAGGCATGATTGGACACTTACATTTAAAAACACATGATTTAGAC
    GCAGCATATCAATGTTACATTGAACAATTAGGATTCCAACATGTGTCTGATTTTCCACGT
    GCGCTATTTATGTCAACGAATCATTATCACCATCATATTGCTGCTAATACTTGGCAATCC
    AATCAAGCAAGACAAAACAATTCACAAAGTTATGGATTAACACATGTTGATATTTATCAA
    CCCGACGCCTCAACACATACATTTACGGCACCAGAAGGGTTTGACATCACAGTTCATTCA
    AATACTGACCTTGTTCCAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    807

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2533 [new locus tag: SACOL_RS13270 ]
  • symbol: SACOL2533
  • description: glyoxalase
  • length: 268
  • theoretical pI: 5.74646
  • theoretical MW: 30059.2
  • GRAVY: -0.438433

⊟Function[edit | edit source]

  • TIGRFAM:
    3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 45.6)
    Metabolism Energy metabolism Other catechol 2,3 dioxygenase (TIGR03211; EC 1.13.11.2; HMM-score: 41.1)
    and 4 more
    Metabolism Energy metabolism Other 2,3-dihydroxybiphenyl 1,2-dioxygenase (TIGR03213; EC 1.13.11.39; HMM-score: 27.5)
    methylmalonyl-CoA epimerase (TIGR03081; EC 5.1.99.1; HMM-score: 17.1)
    Metabolism Energy metabolism Other lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 15.4)
    Metabolism Central intermediary metabolism Amino sugars lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 15.4)
  • TheSEED  :
    • Glyoxalase family protein
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Glyoxalase family protein
  • PFAM:
    Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 63.8)
    and 3 more
    CppA_N; CppA N-terminal (PF14506; HMM-score: 14.4)
    Glyoxalase_3; Glyoxalase-like domain (PF13468; HMM-score: 12.7)
    Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003851
    • TAT(Tat/SPI): 0.000332
    • LIPO(Sec/SPII): 0.000611
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTTVLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGAGDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGWQGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTWQSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 37 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2533&oldid=59780"