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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2461 [new locus tag: SAUSA300_RS13650 ]
  • pan locus tag?: SAUPAN006167000
  • symbol: SAUSA300_2461
  • pan gene symbol?:
  • synonym:
  • product: glyoxalase family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2461 [new locus tag: SAUSA300_RS13650 ]
  • symbol: SAUSA300_2461
  • product: glyoxalase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2657553..2658359
  • length: 807
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCATTTCACGATAAAACAGCAACACAAGTGACAAACATTGTACTGAACGTAAGAGAC
    TTAGACTTAATGACAACATTTTACAAAAACATTTTAGGACTATCCGTTAAAAGTTCTGAC
    GATAATACAACCGTATTATCTGTTGGGACTGGTGGCCATACTCTAACGTTACATTTATTA
    GAAGACGGCCGTCAGACTTCCCCACGTGAAGCAGGGCTTTTTCATATAGCATTTTTATTA
    CCAACTACTGAGGATCTAGCTAACTTCTTATATTTCGTGGCACAAAAAAATATGGGCATC
    GGCGCTGGTGATCATTTAGTAAGTGAAGCTTTATATTTCAACGACCCCGAAGGTAATGGT
    ATTGAAGTCTATCGCGATAGACCTTCATCTTCATGGGAATGGCAAAATGGCAAAGTTAAA
    ATGGATACTTTAGAAGTTGATAGCCAAACCTTATTAACACATCGTACTGATGAAGGTTGG
    CAAGGAATGCCAGCAAAAGGCATGATTGGACACTTACATTTAAAAACACATGATTTAGAC
    GCAGCATATCAATGTTACATTGAACAATTAGGATTCCAACATGTGTCTGATTTTCCACGT
    GCGCTATTTATGTCAACGAATCATTATCACCATCATATTGCTGCTAATACTTGGCAATCC
    AATCAAGCAAGACAAAACAATTCACAAAGTTATGGATTAACACATGTTGATATTTATCAA
    CCCGACGCCTCAACACATACATTTACGGCACCAGAAGGGTTTGACATCACAGTTCATTCA
    AATACTGACCTTGTTCCAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    807

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2461 [new locus tag: SAUSA300_RS13650 ]
  • symbol: SAUSA300_2461
  • description: glyoxalase family protein
  • length: 268
  • theoretical pI: 5.74646
  • theoretical MW: 30059.2
  • GRAVY: -0.438433

Function[edit | edit source]

  • reaction:
    EC 1.13.11.39?  ExPASy
    Biphenyl-2,3-diol 1,2-dioxygenase Biphenyl-2,3-diol + O2 = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
  • TIGRFAM:
    3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 45.6)
    Metabolism Energy metabolism Other catechol 2,3 dioxygenase (TIGR03211; EC 1.13.11.2; HMM-score: 41.1)
    and 4 more
    Metabolism Energy metabolism Other 2,3-dihydroxybiphenyl 1,2-dioxygenase (TIGR03213; EC 1.13.11.39; HMM-score: 27.5)
    methylmalonyl-CoA epimerase (TIGR03081; EC 5.1.99.1; HMM-score: 17.1)
    Metabolism Energy metabolism Other lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 15.4)
    Metabolism Central intermediary metabolism Amino sugars lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 15.4)
  • TheSEED  :
    • Glyoxalase family protein
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Glyoxalase family protein
  • PFAM:
    Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 63.8)
    and 3 more
    CppA_N; CppA N-terminal (PF14506; HMM-score: 14.4)
    Glyoxalase_3; Glyoxalase-like domain (PF13468; HMM-score: 12.7)
    Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 12)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003851
    • TAT(Tat/SPI): 0.000332
    • LIPO(Sec/SPII): 0.000611
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTTVLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGAGDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGWQGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTWQSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]