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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS13650 [old locus tag: SAUSA300_2461 ]
- pan locus tag?: SAUPAN006167000
- symbol: SAUSA300_RS13650
- pan gene symbol?: —
- synonym:
- product: glyoxalase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS13650 [old locus tag: SAUSA300_2461 ]
- symbol: SAUSA300_RS13650
- product: glyoxalase
- replicon: chromosome
- strand: +
- coordinates: 2657553..2658359
- length: 807
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2657553..2658359) NCBI
- BioCyc: SAUSA300_RS13650 BioCyc
- MicrobesOnline: see SAUSA300_2461
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGGCATTTCACGATAAAACAGCAACACAAGTGACAAACATTGTACTGAACGTAAGAGAC
TTAGACTTAATGACAACATTTTACAAAAACATTTTAGGACTATCCGTTAAAAGTTCTGAC
GATAATACAACCGTATTATCTGTTGGGACTGGTGGCCATACTCTAACGTTACATTTATTA
GAAGACGGCCGTCAGACTTCCCCACGTGAAGCAGGGCTTTTTCATATAGCATTTTTATTA
CCAACTACTGAGGATCTAGCTAACTTCTTATATTTCGTGGCACAAAAAAATATGGGCATC
GGCGCTGGTGATCATTTAGTAAGTGAAGCTTTATATTTCAACGACCCCGAAGGTAATGGT
ATTGAAGTCTATCGCGATAGACCTTCATCTTCATGGGAATGGCAAAATGGCAAAGTTAAA
ATGGATACTTTAGAAGTTGATAGCCAAACCTTATTAACACATCGTACTGATGAAGGTTGG
CAAGGAATGCCAGCAAAAGGCATGATTGGACACTTACATTTAAAAACACATGATTTAGAC
GCAGCATATCAATGTTACATTGAACAATTAGGATTCCAACATGTGTCTGATTTTCCACGT
GCGCTATTTATGTCAACGAATCATTATCACCATCATATTGCTGCTAATACTTGGCAATCC
AATCAAGCAAGACAAAACAATTCACAAAGTTATGGATTAACACATGTTGATATTTATCAA
CCCGACGCCTCAACACATACATTTACGGCACCAGAAGGGTTTGACATCACAGTTCATTCA
AATACTGACCTTGTTCCAGAAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS13650 [old locus tag: SAUSA300_2461 ]
- symbol: SAUSA300_RS13650
- description: glyoxalase
- length: 268
- theoretical pI: 5.74646
- theoretical MW: 30059.2
- GRAVY: -0.438433
⊟Function[edit | edit source]
- reaction: EC 1.13.11.39? ExPASyBiphenyl-2,3-diol 1,2-dioxygenase Biphenyl-2,3-diol + O2 = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
- TIGRFAM: 3,4-dihydroxyphenylacetate 2,3-dioxygenase (TIGR02295; EC 1.13.11.15; HMM-score: 45.6)Energy metabolism Other catechol 2,3 dioxygenase (TIGR03211; EC 1.13.11.2; HMM-score: 41.1)and 4 moreEnergy metabolism Other 2,3-dihydroxybiphenyl 1,2-dioxygenase (TIGR03213; EC 1.13.11.39; HMM-score: 27.5)methylmalonyl-CoA epimerase (TIGR03081; EC 5.1.99.1; HMM-score: 17.1)Energy metabolism Other lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 15.4)Central intermediary metabolism Amino sugars lactoylglutathione lyase (TIGR00068; EC 4.4.1.5; HMM-score: 15.4)
- TheSEED: see SAUSA300_2461
- PFAM: Glyoxalase (CL0104) Glyoxalase; Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily (PF00903; HMM-score: 63.8)and 3 moreCppA_N; CppA N-terminal (PF14506; HMM-score: 14.4)Glyoxalase_3; Glyoxalase-like domain (PF13468; HMM-score: 12.7)Glyoxalase_2; Glyoxalase-like domain (PF12681; HMM-score: 12)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003851
- TAT(Tat/SPI): 0.000332
- LIPO(Sec/SPII): 0.000611
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446786729 NCBI
- RefSeq: WP_000863985 NCBI
- UniProt: see SAUSA300_2461
⊟Protein sequence[edit | edit source]
- MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTTVLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGAGDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGWQGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTWQSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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