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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2539 [new locus tag: SACOL_RS13300 ]
  • pan locus tag?: SAUPAN006178000
  • symbol: srtA
  • pan gene symbol?: srtA
  • synonym:
  • product: sortase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2539 [new locus tag: SACOL_RS13300 ]
  • symbol: srtA
  • product: sortase
  • replicon: chromosome
  • strand: -
  • coordinates: 2600008..2600628
  • length: 621
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAAAAATGGACAAATCGATTAATGACAATCGCTGGTGTGGTACTTATCCTAGTGGCA
    GCATATTTGTTTGCTAAACCACATATCGATAATTATCTTCACGATAAAGATAAAGATGAA
    AAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGTAAAGATAAAAAGCAGCAA
    GCTAAACCTCAAATTCCGAAAGATAAATCGAAAGTGGCAGGCTATATTGAAATTCCAGAT
    GCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCTGAACAATTAAATAGAGGT
    GTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAATATTTCAATTGCAGGACAC
    ACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAAGCAGCCAAAAAAGGTAGT
    ATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAAATGACAAGTATAAGAGAT
    GTTAAGCCTACAGATGTAGGAGTTCTAGATGAACAAAAAGGTAAAGATAAACAATTAACA
    TTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGGGAAAAACGTAAAATCTTT
    GTAGCTACAGAAGTCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    621

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2539 [new locus tag: SACOL_RS13300 ]
  • symbol: SrtA
  • description: sortase
  • length: 206
  • theoretical pI: 9.32932
  • theoretical MW: 23540.7
  • GRAVY: -0.792233

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other sortase (TIGR01076; EC 3.4.22.-; HMM-score: 163.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase (TIGR01076; EC 3.4.22.-; HMM-score: 163.4)
    and 3 more
    sortase, marine proteobacterial type (TIGR03784; HMM-score: 34.8)
    Cell structure Cell envelope Other sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.9)
  • TheSEED  :
    • LPXTG specific sortase A
    Cell Wall and Capsule Gram-Positive cell wall components Sortase  LPXTG specific sortase A
    and 1 more
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  LPXTG specific sortase A
  • PFAM:
    no clan defined Sortase; Sortase family (PF04203; HMM-score: 74.6)
    and 2 more
    DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 16.7)
    DUF3959; Protein of unknown function (DUF3959) (PF13105; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.87
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.670618
    • TAT(Tat/SPI): 0.002414
    • LIPO(Sec/SPII): 0.091978
    • Cleavage Site: CS pos: 25-26. LFA-KP. Pr: 0.4199
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Changsheng Lu, Jie Zhu, Yun Wang, Aiko Umeda, Roshani B Cowmeadow, Eric Lai, Gabrielle N Moreno, Maria D Person, Zhiwen Zhang
Staphylococcus aureus sortase A exists as a dimeric protein in vitro.
Biochemistry: 2007, 46(32);9346-54
[PubMed:17658894] [WorldCat.org] [DOI] (P p)

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