PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2316 [new locus tag: SA_RS13275 ]
• pan locus tag^?: SAUPAN006178000
• symbol: srtA
• pan gene symbol^?: srtA
• synonym:
• product: sortase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2316 [new locus tag: SA_RS13275 ]
• symbol: srtA
• product: sortase
• replicon: chromosome
• strand: -
• coordinates: 2600886..2601506
• length: 621
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125243 NCBI
• RefSeq: NP_375640 NCBI
• BioCyc: see SA_RS13275
• MicrobesOnline: 104666 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAATGGACAAATCGATTAATGACAATCGCTGGTGTAGTACTTATCCTAGTGGCA
  GCATATTTGTTTGCTAAACCACATATCGATAATTATCTTCACGATAAAGATAAAGATGAA
  AAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGTAAAGACAATAAGCAGCAA
  GCTAAACCTCAAATTCCGAAAGATAAATCAAAAGTGGCAGGCTATATTGAAATTCCAGAT
  GCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCTGAACAATTAAATAGAGGT
  GTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAATATTTCAATTGCAGGACAC
  ACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAAGCAGCCAAAAAAGGTAGT
  ATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAAATGACAAGTATAAGAGAT
  GTTAAGCCAACAGATGTAGAAGTTCTAGATGAACAAAAAGGTAAAGATAAACAATTAACA
  TTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGGGAAAAACGTAAAATCTTT
  GTAGCTACAGAAGTCAAATAA
  60
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  621
  

⊟Protein

⊟General

• locus tag: SA2316 [new locus tag: SA_RS13275 ]
• symbol: SrtA
• description: sortase
• length: 206
• theoretical pI: 8.80677
• theoretical MW: 23598.7
• GRAVY: -0.80534

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Other sortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)
  and 3 more
  sortase, marine proteobacterial type (TIGR03784; HMM-score: 35.6)
  Cell structure Cell envelope Other sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)
• TheSEED  :
  • LPXTG specific sortase A
  Cell Wall and Capsule Gram-Positive cell wall components Sortase  LPXTG specific sortase A
  and 1 more
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  LPXTG specific sortase A
• PFAM:
  no clan defined Sortase; Sortase family (PF04203; HMM-score: 76)
  and 2 more
  DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 17.2)
  DUF3959; Protein of unknown function (DUF3959) (PF13105; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.87
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 7
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 25 aa
  • SP(Sec/SPI): 0.681174
  • TAT(Tat/SPI): 0.002378
  • LIPO(Sec/SPII): 0.090972
  • Cleavage Site: CS pos: 25-26. LFA-KP. Pr: 0.4245
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15928107 NCBI
• RefSeq: NP_375640 NCBI
• UniProt: A0A0H3JNM2 UniProt

⊟Protein sequence

• MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Matthew L Bentley, Helena Gaweska, Joseph M Kielec, Dewey G McCafferty
Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA.
J Biol Chem: 2007, 282(9);6571-81
[PubMed:17200112] [WorldCat.org] [DOI] (P p)Hidehiko Hirakawa, Suguru Ishikawa, Teruyuki Nagamune
Design of Ca2+-independent Staphylococcus aureus sortase A mutants.
Biotechnol Bioeng: 2012, 109(12);2955-61
[PubMed:22729808] [WorldCat.org] [DOI] (I p)