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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13275 [old locus tag: SA2316 ]
  • pan locus tag?: SAUPAN006178000
  • symbol: SA_RS13275
  • pan gene symbol?: srtA
  • synonym:
  • product: class A sortase SrtA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13275 [old locus tag: SA2316 ]
  • symbol: SA_RS13275
  • product: class A sortase SrtA
  • replicon: chromosome
  • strand: -
  • coordinates: 2600886..2601506
  • length: 621
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2600886..2601506) NCBI
  • BioCyc: SA_RS13275 BioCyc
  • MicrobesOnline: see SA2316

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAAAAATGGACAAATCGATTAATGACAATCGCTGGTGTAGTACTTATCCTAGTGGCA
    GCATATTTGTTTGCTAAACCACATATCGATAATTATCTTCACGATAAAGATAAAGATGAA
    AAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGTAAAGACAATAAGCAGCAA
    GCTAAACCTCAAATTCCGAAAGATAAATCAAAAGTGGCAGGCTATATTGAAATTCCAGAT
    GCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCTGAACAATTAAATAGAGGT
    GTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAATATTTCAATTGCAGGACAC
    ACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAAGCAGCCAAAAAAGGTAGT
    ATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAAATGACAAGTATAAGAGAT
    GTTAAGCCAACAGATGTAGAAGTTCTAGATGAACAAAAAGGTAAAGATAAACAATTAACA
    TTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGGGAAAAACGTAAAATCTTT
    GTAGCTACAGAAGTCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    621

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS13275 [old locus tag: SA2316 ]
  • symbol: SA_RS13275
  • description: class A sortase SrtA
  • length: 206
  • theoretical pI: 8.80677
  • theoretical MW: 23598.7
  • GRAVY: -0.80534

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other sortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase (TIGR01076; EC 3.4.22.-; HMM-score: 164.8)
    and 3 more
    sortase, marine proteobacterial type (TIGR03784; HMM-score: 35.6)
    Cell structure Cell envelope Other sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 30.4)
  • TheSEED: see SA2316
  • PFAM:
    no clan defined Sortase; Sortase family (PF04203; HMM-score: 76)
    and 2 more
    DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 17.2)
    DUF3959; Protein of unknown function (DUF3959) (PF13105; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.87
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 25 aa
    • SP(Sec/SPI): 0.681174
    • TAT(Tat/SPI): 0.002378
    • LIPO(Sec/SPII): 0.090972
    • Cleavage Site: CS pos: 25-26. LFA-KP. Pr: 0.4245
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQYDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVSFAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRDVKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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