PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS01260 [old locus tag: SACOL0249 ]
• pan locus tag^?: SAUPAN001143000
• symbol: SACOL_RS01260
• pan gene symbol^?: bglR
• synonym:
• product: GntR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01260 [old locus tag: SACOL0249 ]
• symbol: SACOL_RS01260
• product: GntR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 289279..289983
• length: 705
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (289279..289983) NCBI
• BioCyc: SACOL_RS01260 BioCyc
• MicrobesOnline: see SACOL0249

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGTTAAAGTATGAACATATTGCTAAGCAACTTAATGCGTTTATACATCAATCTAATTTC
  AAACCCGGTGATAAATTGCCAAGCGTGACGCAATTAAAAGAACGTTATCAAGTAAGTAAG
  AGTACTATCATTAAAGCATTAGGCTTATTGGAACAAGATGGTTTGATCTATCAAGCACAA
  GGCAGTGGTATTTATGTGAGAAATATTGCTGATGCCAATCGTATCAACGTCTTTAAGACT
  AATGGTTTCTCTAAAAGTTTAGGTGAACACCGAATGACAAGTAAGGTACTTGTTTTTAAG
  GAGATTGCAACGCCACCTAAATCTGTACAAGATGAGCTCCAATTAAATGCAGATGATACC
  GTCTACTATTTAGAGCGATTAAGATTCGTGGACGATGATGTTTTATGTATCGAATATTCT
  TATTATCATAAAGAAATCGTGAAATATTTAAATGATGATATTGCTAAGGGCTCTATCTTC
  GACTATTTAGAATCAAACATGAAACTTCGTATTGGTTTTTCAGATATTTTCTTTAATGTA
  GATCAACTCACTTCAAGTGAAGCTTCATTACTACAATTGTCTACAGGTGAACCATGTTTA
  CGTTACCACCAGACTTTTTATACAATGACTGGCAAACCCTTTGATTCATCTGACATCGTA
  TTTCATTATCGTCATGCACAGTTTTATATTCCTAGTAAAAAGTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS01260 [old locus tag: SACOL0249 ]
• symbol: SACOL_RS01260
• description: GntR family transcriptional regulator
• length: 234
• theoretical pI: 7.67897
• theoretical MW: 27013.6
• GRAVY: -0.359829

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 112.2)
  Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 90.1)
  and 10 more
  Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 77.2)
  Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 77.2)
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 66.7)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 66.7)
  phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 27.8)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 17.4)
  Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 17.4)
  Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 17.4)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 12.9)
  Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 12.1)
• TheSEED: see SACOL0249
• PFAM:
  UTRA (CL0122) UTRA; UTRA domain (PF07702; HMM-score: 91.7)
  and 11 more
  HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 61.1)
  HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 22.8)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 21.7)
  Rrf2; Transcriptional regulator (PF02082; HMM-score: 16.8)
  HTH_11; HTH domain (PF08279; HMM-score: 16)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 15.4)
  MarR; MarR family (PF01047; HMM-score: 15.3)
  MarR_2; MarR family (PF12802; HMM-score: 15.2)
  HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 14.2)
  HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 13.1)
  RPA_C; Replication protein A C terminal (PF08784; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by BglR*, TF important in Beta-glucosides utilization: see SACOL0249

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00433
  • TAT(Tat/SPI): 0.000361
  • LIPO(Sec/SPII): 0.000474
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446845073 NCBI
• RefSeq: WP_000922329 NCBI
• UniProt: see SACOL0249

⊟Protein sequence

• MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTIIKALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEIATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIFDYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSDIVFHYRHAQFYIPSKK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL0249
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: BglR* see SACOL0249

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications