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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS03320 [old locus tag: SACOL0643 ]
  • pan locus tag?: SAUPAN002442000
  • symbol: SACOL_RS03320
  • pan gene symbol?: tspA
  • synonym:
  • product: transposase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS03320 [old locus tag: SACOL0643 ]
  • symbol: SACOL_RS03320
  • product: transposase
  • replicon: chromosome
  • strand: +
  • coordinates: 674101..675510
  • length: 1410
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (674101..675510) NCBI
  • BioCyc: SACOL_RS03320 BioCyc
  • MicrobesOnline: see SACOL0643

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGTATTGACATGTATTTAGACAGATCTCGAAACCAAGCTTCAAGTGTGGGGAATTTG
    AGTCAAACAATGAATTCAAATTATGATGCGTTGGAAAAAGCAATTACTCAATTTATTAAT
    GATGATGCGCTTAAAGGGAAAGCGTATACGTCAGCTAAGCAATTTTTTAGTACGGTGTTA
    ATTCCATTATCAACAAGTATGAAAACATTGAGTGATTTAACGAAGCAAGCTTGCGATAAT
    TTTGTGTCACGTTATACGAGTGAGGTTGATAGCATATCTTTAAAAGAATCAGAGCTTGAA
    GAAGATATCAGATCATTAAGTCAACAAATTACGCGATATGAAAATTTGAATAACAATTTG
    AAAAAGCATGCTTCCGATAATCAGCAAGCCATTTCATCGAACCAACAAATAATACGAACA
    TTAGGTCAACAAAAACATGAATTAGAAGAGAAGCTACGCAAATTGCGTGAGTTTAATCAA
    AAATCACCAGAAATATTTAAAGAAGTTGAAGAATTTCAAAAAATTGTCCAACAAGGACTT
    ACCCAAGCGCAGAATTTTTGGAACTTTTCAACAAATCAATTTAATATTCCTTCAGGTAAA
    GAACTTGATTGGGCCAAAGCAAGTCATGAAAAATATTTGAAAGTTGCTATGGGGAAAATT
    GAACATAAAGCAGAGAAAGAAACTTTAAATAAAGCAGACTTTGCTGTTATAAAGGCATAT
    GCCAAAGAACATCCAGAAGACGATATCCCGAAAAGTATATTGAAATATATAAATGACAAT
    AAAGACAGTATTAAAAGAGATATAGGATTAGATATTACGTCAACACTTTTAGAGCAAGGC
    GGTATAAATGCAAGTAAATTCGGTGTATTTATCAATACAGCAGGTGGAGTGAAAGGCCCA
    GCAGGTCCAAATTCATTTGTGGAAGTCAAACGTACATCAGGTAATGTGTTTATAGAAAAT
    GGTAGTAAATTTGCAAAAGGCGGAAAATACCTAGGTAAAGGTGTTGCTGGTGTAGGATTT
    GGTATAGGTATGTATGATGACCTTGCAAATGATGATAAAACATTTGGAGAGGCGTTGTCG
    CATAATGGTATGACGCTTGCAGCTGGATCTGCAGGGACAGCAGTTGGAGCTGGATTAGCG
    ACTTTTGTTTTAGGAAGTAATCCAGTAGGATGGGTGATTTTAGCAGGTTTGGCTATGAGT
    ACAGTATTTGCATTAGGAACAGATTTAATTTATCAAAATAATATTTTTGGATTAAAAGAT
    AAAGTAGACTGGGTAGGGCATAAAATCGATAATAGTATAGATGTTGTTAAAAAAACTACA
    GAAAAATCCATGGATAGTGTTGGGAATGCTGTTAGTGAAGCTAAAAATATTATTAGCAAT
    CATATAAATCCAATGAAATGGGCGTGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1410

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS03320 [old locus tag: SACOL0643 ]
  • symbol: SACOL_RS03320
  • description: transposase
  • length: 469
  • theoretical pI: 7.294
  • theoretical MW: 51610.9
  • GRAVY: -0.42516

⊟Function[edit | edit source]

  • TIGRFAM:
    SH3 domain protein (TIGR04211; HMM-score: 15.3)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 14)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 14)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 12.9)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 12.9)
    and 2 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 7.1)
    two transmembrane protein (TIGR04527; HMM-score: 5.4)
  • TheSEED: see SACOL0643
  • PFAM:
    EsxAB (CL0352) LXG; LXG domain of WXG superfamily (PF04740; HMM-score: 37.8)
    and 32 more
    no clan defined DUF3450; Protein of unknown function (DUF3450) (PF11932; HMM-score: 19.9)
    Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 17.3)
    Concanavalin (CL0004) Laminin_II; Laminin Domain II (PF06009; HMM-score: 17.2)
    Tropomyosin-lke (CL0452) Tropomyosin; Tropomyosin (PF00261; HMM-score: 16.5)
    no clan defined DegS; Sensor protein DegS (PF05384; HMM-score: 15.1)
    Apolipoprotein (CL0725) ApoLp-III; Apolipophorin-III precursor (apoLp-III) (PF07464; HMM-score: 13.9)
    Ferritin (CL0044) DUF892; Domain of unknown function (DUF892) (PF05974; HMM-score: 13.7)
    no clan defined CALCOCO1; Calcium binding and coiled-coil domain (CALCOCO1) like (PF07888; HMM-score: 13.3)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 11.9)
    APG6_N; Apg6 coiled-coil region (PF17675; HMM-score: 11.9)
    DUF5740; Family of unknown function (DUF5740) (PF19011; HMM-score: 11.5)
    Acetyltransf_11; Udp N-acetylglucosamine O-acyltransferase; Domain 2 (PF13720; HMM-score: 11.4)
    DUF4200; Domain of unknown function (DUF4200) (PF13863; HMM-score: 11.1)
    TPR (CL0020) COG6_N; Conserved oligomeric complex COG6, N-terminal (PF06419; HMM-score: 11)
    no clan defined ADIP; Afadin- and alpha -actinin-Binding (PF11559; HMM-score: 10.7)
    GT-B (CL0113) FUT8_N_cat; Alpha-(1,6)-fucosyltransferase N- and catalytic domains (PF19745; HMM-score: 10.7)
    HTH (CL0123) RBD_LARS1; Leucine--tRNA ligase, RagD-binding domain (PF24810; HMM-score: 10.4)
    CorA-like (CL0788) CorA; CorA-like Mg2+ transporter protein (PF01544; HMM-score: 10.1)
    no clan defined ADAMTSL1; ADAMTSL1 domain (PF24484; HMM-score: 10.1)
    Mis12; Mis12 protein (PF05859; HMM-score: 9.9)
    Laminin_I; Laminin Domain I (PF06008; HMM-score: 9.6)
    HTH (CL0123) Vir_act_alpha_C; Virulence activator alpha C-term (PF10400; HMM-score: 9.5)
    no clan defined CC4_CEP85; Centrosomal protein of 85 kDa-like, CC4 coiled-coil domain (PF24555; HMM-score: 9.1)
    NNH2; NACHT N-terminal Helical domain 2 (PF22734; HMM-score: 9)
    V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 8.6)
    DUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 8.3)
    BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 8.2)
    crAss001_48; crAss001_48 related protein (PF21825; HMM-score: 8.2)
    DivIVA; DivIVA protein (PF05103; HMM-score: 8.1)
    TMPIT; TMPIT-like protein (PF07851; HMM-score: 8.1)
    TSC22; TSC-22/dip/bun family (PF01166; HMM-score: 8)
    Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 6.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.0349
    • Cell wall & surface Score: 0.0022
    • Extracellular Score: 0.9628
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006641
    • TAT(Tat/SPI): 0.001543
    • LIPO(Sec/SPII): 0.000945
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDALKGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEEDIRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQKSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMGKIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTLLEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKGVAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWVILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNAVSEAKNIISNHINPMKWAW

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0643
  • protein localization: see SACOL0643
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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