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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00584
  • pan locus tag?: SAUPAN002442000
  • symbol: SAOUHSC_00584
  • pan gene symbol?: tspA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00584
  • symbol: SAOUHSC_00584
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 585426..586835
  • length: 1410
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGTATTGACATGTATTTAGACAGATCTCGAAACCAAGCTTCAAGTGTGGGGAATTTG
    AGTCAAACAATGAATTCAAATTATGATGCGTTGGAAAAAGCAATTACTCAATTTATTAAT
    GATGATGCGCTTAAAGGGAAAGCGTATACGTCAGCTAAGCAATTTTTTAGTACGGTGTTA
    ATTCCATTATCAACAAGTATGAAAACATTGAGTGATTTAACGAAGCAAGCTTGCGATAAT
    TTTGTGTCACGTTATACGAGTGAGGTTGATAGCATATCTTTAAAAGAATCAGAGCTTGAA
    GAAGATATCAGATCATTAAGTCAACAAATTACGCGATATGAAAATTTGAATAACAATTTG
    AAAAAGCATGCTTCCGATAATCAGCAAGCCATTTCATCGAACCAACAAATAATACGAACA
    TTAGGTCAACAAAAACATGAATTAGAAGAGAAGCTACGCAAATTGCGTGAGTTTAATCAA
    AAATCACCAGAAATATTTAAAGAAGTTGAAGAATTTCAAAAAATTGTCCAACAAGGACTT
    ACCCAAGCGCAGAATTTTTGGAACTTTTCAACAAATCAATTTAATATTCCTTCAGGTAAA
    GAACTTGATTGGGCCAAAGCAAGTCATGAAAAATATTTGAAAGTTGCTATGGGGAAAATT
    GAACATAAAGCAGAGAAAGAAACTTTAAATAAAGCAGACTTTGCTGTTATAAAGGCATAT
    GCCAAAGAACATCCAGAAGACGATATCCCGAAAAGTATATTGAAATATATAAATGACAAT
    AAAGACAGTATTAAAAGAGATATAGGATTAGATATTACGTCAACACTTTTAGAGCAAGAC
    GGTATAAATGCAAGTAAATTCGGTGTATTTATCAATACAGCAGGTGGAGTGAAAGGCCCA
    GCAGGTCCAAATTCATTTGTGGAAGTCAAACGTACATCAGGTAATGTGTTTATAGAAAAT
    GGTAGTAAATTTGCAAAAGGCGGAAAATACCTAGGTAAAGGTGTTGCTGGTGTAGGATTT
    GGTATAGGTATGTATGATGACCTTGCAAATGATGATAAAACATTTGGAGAGGCGTTGTCG
    CATAATGGTATGACGCTTGCAGCTGGATCTGCAGGGACAGCAGTTGGAGCTGGATTAGCG
    ACTTTTGTTTTAGGAAGTAATCCAGTAGGATGGGTGATTTTAGCAGGTTTGGCTATGAGT
    ACAGTATTTGCATTAGGAACAGATTTAATTTATCAAAATAATATTTTTGGATTAAAAGAT
    AAAGTAGACTGGGTAGGGCATAAAATCGATAATAGTATAGATGTTGTTAAAAAAACTACA
    GAAAAATCCATGGATAGTGTTGGGAATGCTGTTAGTGAAGCTAAAAATATTATTAGCAAT
    CATATAAATCCAATGAAATGGGCGTGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1410

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00584
  • symbol: SAOUHSC_00584
  • description: hypothetical protein
  • length: 469
  • theoretical pI: 6.97557
  • theoretical MW: 51668.9
  • GRAVY: -0.43177

⊟Function[edit | edit source]

  • TIGRFAM:
    SH3 domain protein (TIGR04211; HMM-score: 15.3)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 14)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 14)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 12.9)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 12.9)
    and 3 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 7.1)
    two transmembrane protein (TIGR04527; HMM-score: 5.4)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage tail tape measure protein, lambda family (TIGR01541; HMM-score: 4.2)
  • TheSEED  :
    • hypothetical fig|282458.1.peg.573 homolog
  • PFAM:
    EsxAB (CL0352) LXG; LXG domain of WXG superfamily (PF04740; HMM-score: 37.8)
    and 32 more
    no clan defined DUF3450; Protein of unknown function (DUF3450) (PF11932; HMM-score: 19.9)
    Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 17.3)
    Concanavalin (CL0004) Laminin_II; Laminin Domain II (PF06009; HMM-score: 17.2)
    Tropomyosin-lke (CL0452) Tropomyosin; Tropomyosin (PF00261; HMM-score: 16.5)
    no clan defined DegS; Sensor protein DegS (PF05384; HMM-score: 15.1)
    Apolipoprotein (CL0725) ApoLp-III; Apolipophorin-III precursor (apoLp-III) (PF07464; HMM-score: 13.9)
    Ferritin (CL0044) DUF892; Domain of unknown function (DUF892) (PF05974; HMM-score: 13.7)
    no clan defined CALCOCO1; Calcium binding and coiled-coil domain (CALCOCO1) like (PF07888; HMM-score: 13.3)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 11.9)
    APG6_N; Apg6 coiled-coil region (PF17675; HMM-score: 11.9)
    DUF5740; Family of unknown function (DUF5740) (PF19011; HMM-score: 11.5)
    Acetyltransf_11; Udp N-acetylglucosamine O-acyltransferase; Domain 2 (PF13720; HMM-score: 11.4)
    DUF4200; Domain of unknown function (DUF4200) (PF13863; HMM-score: 11.1)
    ADIP; Afadin- and alpha -actinin-Binding (PF11559; HMM-score: 10.7)
    GT-B (CL0113) FUT8_N_cat; Alpha-(1,6)-fucosyltransferase N- and catalytic domains (PF19745; HMM-score: 10.7)
    HTH (CL0123) RBD_LARS1; Leucine--tRNA ligase, RagD-binding domain (PF24810; HMM-score: 10.4)
    TPR (CL0020) COG6_N; Conserved oligomeric complex COG6, N-terminal (PF06419; HMM-score: 10.2)
    CorA-like (CL0788) CorA; CorA-like Mg2+ transporter protein (PF01544; HMM-score: 10.1)
    no clan defined ADAMTSL1; ADAMTSL1 domain (PF24484; HMM-score: 10.1)
    Mis12; Mis12 protein (PF05859; HMM-score: 9.9)
    Laminin_I; Laminin Domain I (PF06008; HMM-score: 9.6)
    HTH (CL0123) Vir_act_alpha_C; Virulence activator alpha C-term (PF10400; HMM-score: 9.5)
    no clan defined NNH2; NACHT N-terminal Helical domain 2 (PF22734; HMM-score: 9.1)
    CC4_CEP85; Centrosomal protein of 85 kDa-like, CC4 coiled-coil domain (PF24555; HMM-score: 9.1)
    V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 8.9)
    DUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 8.3)
    BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 8.2)
    crAss001_48; crAss001_48 related protein (PF21825; HMM-score: 8.2)
    DivIVA; DivIVA protein (PF05103; HMM-score: 8.1)
    TMPIT; TMPIT-like protein (PF07851; HMM-score: 8.1)
    TSC22; TSC-22/dip/bun family (PF01166; HMM-score: 8)
    Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 6.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.025
    • Cell wall & surface Score: 0.002
    • Extracellular Score: 0.9729
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006641
    • TAT(Tat/SPI): 0.001543
    • LIPO(Sec/SPII): 0.000945
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDALKGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEEDIRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQKSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMGKIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTLLEQDGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKGVAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWVILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNAVSEAKNIISNHINPMKWAW

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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