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NCBI: 03-AUG-2016

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00584
  • pan locus tag?: SAUPAN002442000
  • symbol: SAOUHSC_00584
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00584
  • symbol: SAOUHSC_00584
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 585426..586835
  • length: 1410
  • essential: no DEG other strains

⊟Accession numbers[edit source | edit]

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGTATTGACATGTATTTAGACAGATCTCGAAACCAAGCTTCAAGTGTGGGGAATTTG
    AGTCAAACAATGAATTCAAATTATGATGCGTTGGAAAAAGCAATTACTCAATTTATTAAT
    GATGATGCGCTTAAAGGGAAAGCGTATACGTCAGCTAAGCAATTTTTTAGTACGGTGTTA
    ATTCCATTATCAACAAGTATGAAAACATTGAGTGATTTAACGAAGCAAGCTTGCGATAAT
    TTTGTGTCACGTTATACGAGTGAGGTTGATAGCATATCTTTAAAAGAATCAGAGCTTGAA
    GAAGATATCAGATCATTAAGTCAACAAATTACGCGATATGAAAATTTGAATAACAATTTG
    AAAAAGCATGCTTCCGATAATCAGCAAGCCATTTCATCGAACCAACAAATAATACGAACA
    TTAGGTCAACAAAAACATGAATTAGAAGAGAAGCTACGCAAATTGCGTGAGTTTAATCAA
    AAATCACCAGAAATATTTAAAGAAGTTGAAGAATTTCAAAAAATTGTCCAACAAGGACTT
    ACCCAAGCGCAGAATTTTTGGAACTTTTCAACAAATCAATTTAATATTCCTTCAGGTAAA
    GAACTTGATTGGGCCAAAGCAAGTCATGAAAAATATTTGAAAGTTGCTATGGGGAAAATT
    GAACATAAAGCAGAGAAAGAAACTTTAAATAAAGCAGACTTTGCTGTTATAAAGGCATAT
    GCCAAAGAACATCCAGAAGACGATATCCCGAAAAGTATATTGAAATATATAAATGACAAT
    AAAGACAGTATTAAAAGAGATATAGGATTAGATATTACGTCAACACTTTTAGAGCAAGAC
    GGTATAAATGCAAGTAAATTCGGTGTATTTATCAATACAGCAGGTGGAGTGAAAGGCCCA
    GCAGGTCCAAATTCATTTGTGGAAGTCAAACGTACATCAGGTAATGTGTTTATAGAAAAT
    GGTAGTAAATTTGCAAAAGGCGGAAAATACCTAGGTAAAGGTGTTGCTGGTGTAGGATTT
    GGTATAGGTATGTATGATGACCTTGCAAATGATGATAAAACATTTGGAGAGGCGTTGTCG
    CATAATGGTATGACGCTTGCAGCTGGATCTGCAGGGACAGCAGTTGGAGCTGGATTAGCG
    ACTTTTGTTTTAGGAAGTAATCCAGTAGGATGGGTGATTTTAGCAGGTTTGGCTATGAGT
    ACAGTATTTGCATTAGGAACAGATTTAATTTATCAAAATAATATTTTTGGATTAAAAGAT
    AAAGTAGACTGGGTAGGGCATAAAATCGATAATAGTATAGATGTTGTTAAAAAAACTACA
    GAAAAATCCATGGATAGTGTTGGGAATGCTGTTAGTGAAGCTAAAAATATTATTAGCAAT
    CATATAAATCCAATGAAATGGGCGTGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1410

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: SAOUHSC_00584
  • symbol: SAOUHSC_00584
  • description: hypothetical protein
  • length: 469
  • theoretical pI: 6.97557
  • theoretical MW: 51668.9
  • GRAVY: -0.43177

⊟Function[edit source | edit]

  • TIGRFAM:
    SH3 domain protein (TIGR04211; HMM-score: 15.3)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02169; HMM-score: 14)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02169; HMM-score: 14)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02168; HMM-score: 12.9)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02168; HMM-score: 12.9)
    and 3 more
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 7.1)
    two transmembrane protein (TIGR04527; HMM-score: 5.4)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage tail tape measure protein, lambda family (TIGR01541; HMM-score: 4.2)
  • TheSEED:  
    hypothetical fig|282458.1.peg.573 homolog
  • PFAM:
    no clan definedLXG; LXG domain of WXG superfamily (PF04740; HMM-score: 48.1)
    and 26 more
    Tropomyosin-lke (CL0452) Tropomyosin_1; Tropomyosin like (PF12718; HMM-score: 17.7)
    no clan definedDUF3450; Protein of unknown function (DUF3450) (PF11932; HMM-score: 17.5)
    Laminin_II; Laminin Domain II (PF06009; HMM-score: 17.3)
    Ferritin (CL0044) DUF892; Domain of unknown function (DUF892) (PF05974; HMM-score: 15.3)
    no clan definedApoLp-III; Apolipophorin-III precursor (apoLp-III) (PF07464; HMM-score: 14.5)
    DegS; Sensor protein DegS (PF05384; HMM-score: 14.3)
    Fe_bilin_red; Ferredoxin-dependent bilin reductase (PF05996; HMM-score: 13.3)
    BCLiA (CL0551) APG6; Autophagy protein Apg6 (PF04111; HMM-score: 13.1)
    no clan definedCALCOCO1; Calcium binding and coiled-coil domain (CALCOCO1) like (PF07888; HMM-score: 13)
    TPR_MLP1_2; TPR/MLP1/MLP2-like protein (PF07926; HMM-score: 12.5)
    Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 11.8)
    DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 11.4)
    CemA; CemA family (PF03040; HMM-score: 10.4)
    BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 10.4)
    Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 10)
    Laminin_I; Laminin Domain I (PF06008; HMM-score: 9.6)
    DUF2570; Protein of unknown function (DUF2570) (PF10828; HMM-score: 8.9)
    TSC22; TSC-22/dip/bun family (PF01166; HMM-score: 8.7)
    Reo_sigma (CL0326) Reo_sigmaC; Reovirus sigma C capsid protein (PF04582; HMM-score: 8.7)
    no clan definedSlyX; SlyX (PF04102; HMM-score: 8.6)
    HTH (CL0123) Vir_act_alpha_C; Virulence activator alpha C-term (PF10400; HMM-score: 8.4)
    no clan definedGCN5L1; GCN5-like protein 1 (GCN5L1) (PF06320; HMM-score: 8.3)
    TMPIT; TMPIT-like protein (PF07851; HMM-score: 8.2)
    Jnk-SapK_ap_N; JNK_SAPK-associated protein-1 (PF09744; HMM-score: 7.8)
    Prominin; Prominin (PF05478; HMM-score: 7.4)
    LMBR1; LMBR1-like membrane protein (PF04791; HMM-score: 4.9)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

⊟Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006641
    • TAT(Tat/SPI): 0.001543
    • LIPO(Sec/SPII): 0.000945
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit source | edit]

⊟Protein sequence[edit source | edit]

  • MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDALKGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEEDIRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQKSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMGKIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTLLEQDGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKGVAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWVILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNAVSEAKNIISNHINPMKWAW

⊟Experimental data[edit source | edit]

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

  • SAOUHSC_00584 no polycistronic organisation predicted

⊟Regulation[edit source | edit]

  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit source | edit]

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