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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00957
  • pan locus tag?: SAUPAN003201000
  • symbol: SAOUHSC_00957
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00957
  • symbol: SAOUHSC_00957
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 932072..932875
  • length: 804
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTTAATGGATCCAAGTTTGATCTTACCTTATTTATGGGTACTTGTCGTTTTAGTATTT
    TTAGAAGGCTTATTAGCAGCAGATAACGCGATTGTTATGGCTGTAATGGTTAAGCACTTA
    CCACCCGAACAACGTAAAAAAGCTTTGTTTTACGGTTTGTTAGGTGCATTTGTATTTAGA
    TTTTTAGCATTATTCTTAATTAGTATTATCGCGAACTTTTGGTTTATTCAAGCTGCAGGA
    GCGGTTTACTTAATTTATATGTCAATCAAAAATCTGTGGCAGTTCTTTAAACACCCAGAA
    ATTGAAAGTCCTGAAGCTGGAGATGATCATCATTATGATGAATCTGGTGAAGAGATTAAA
    GCAAGTAACAAATCATTCTGGGGAACTGTGTTGAAAATAGAATTTGCAGATATCGCATTT
    GCCATTGATTCTATGCTTGCTGCTTTAGCTATTGCTGTAACACTTCCTAAAGTTGGTATT
    CACTTTGGTGGTATGGACTTAGGTCAGTTCGTAGTCATGTTCCTAGGTGGAATGATTGGT
    GTTATTCTAATGCGTTATGCAGCAACATGGTTTGTAGAGCTATTAAACAAATATCCAGGA
    CTTGAAGGTGCAGCCTTCGCGATCGTTGGTTGGGTAGGTGTTAAATTAGTTGTCATGGTA
    TTAGCGCACCCAGACATCGCTGTATTGCCTGAGCACTTCCCACATGGCGTATTATGGCAA
    TCTATTTTCTGGACAGTACTAATTGGATTAGTAATTATCGGTTGGTTAGGTTCAGTTGTT
    AAAAATAAAAAATCGCATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00957
  • symbol: SAOUHSC_00957
  • description: hypothetical protein
  • length: 267
  • theoretical pI: 6.80419
  • theoretical MW: 29702.3
  • GRAVY: 0.792509

⊟Function[edit | edit source]

  • TIGRFAM:
    integral membrane protein, YkoY family (TIGR03716; HMM-score: 297.4)
    and 2 more
    integral membrane protein, YjbE family (TIGR03717; HMM-score: 88.1)
    integral membrane protein, TerC family (TIGR03718; HMM-score: 32.8)
  • TheSEED  :
    • Integral membrane protein TerC family
  • PFAM:
    LysE (CL0292) TerC; Integral membrane protein TerC family (PF03741; HMM-score: 166)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9999
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0001
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.063575
    • TAT(Tat/SPI): 0.000407
    • LIPO(Sec/SPII): 0.036015
  • predicted transmembrane helices (TMHMM): 7

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQRKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIESPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGIHFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVVMVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  5. ↑ Michael Dambach, Melissa Sandoval, Taylor B Updegrove, Vivek Anantharaman, L Aravind, Lauren S Waters, Gisela Storz
    The ubiquitous yybP-ykoY riboswitch is a manganese-responsive regulatory element.
    Mol Cell: 2015, 57(6);1099-1109
    [PubMed:25794618] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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