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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01039
  • pan locus tag?: SAUPAN003316000
  • symbol: SAOUHSC_01039
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01039
  • symbol: SAOUHSC_01039
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1007610..1008236
  • length: 627
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAATTTGGAAAAACAATCGCAGTAGTATTAGCATCTAGTGTCTTGCTTGCAGGATGT
    ACTACGGATAAAAAAGAAATTAAGGCATATTTAAAGCAAGTGGATAAAATTAAAGATGAT
    GAAGAACCAATTAAAACTGTTGGTAAGAAAATTGCTGAATTAGATGAGAAAAAGAAAAAA
    TTAACTGAAGATGTCAATAGTAAAGATACAGCAGTTCGCGGTAAAGCAGTAAAGGATTTA
    ATTAAAAATGCCGATGATCGTCTAAAGGAATTTGAAAAAGAAGAAGACGCAATTAAGAAG
    TCTGAACAAGACTTTAAGAAAGCAAAAAGTCACGTTGATAACATTGATAATGATGTTAAA
    CGTAAAGAAGTAAAACAATTAGATGATGTATTAAAAGAAAAATATAAGTTACACAGTGAT
    TACGCGAAAGCATATAAAAAGGCTGTAAACTCAGAGAAAACATTATTTAAATATTTAAAT
    CAAAATGACGCGACACAACAAGGTGTTAACGAAAAATCAAAAGCAATAGAACAGAACTAT
    AAAAAGTTAAAAGAAGTATCAGATAAGTATACAAAAGTACTAAATAAGGTTGGTAAAGAA
    AAGCAAGACGTTGATCAATTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    627

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01039
  • symbol: SAOUHSC_01039
  • description: hypothetical protein
  • length: 208
  • theoretical pI: 9.96393
  • theoretical MW: 23875.2
  • GRAVY: -1.06587

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 15.4)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 15.4)
    type VII secretion effector, TIGR04197 family (TIGR04197; HMM-score: 12.8)
    and 6 more
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 9)
    SH3 domain protein (TIGR04211; HMM-score: 8.1)
    two transmembrane protein (TIGR04527; HMM-score: 7.7)
    Genetic information processing Mobile and extrachromosomal element functions Plasmid functions conjugative transfer region lipoprotein, TIGR03751 family (TIGR03751; HMM-score: 5.9)
    phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 5.2)
  • TheSEED  :
    • FIG01108153: hypothetical protein
  • PFAM:
    no clan defined YkyA; Putative cell-wall binding lipoprotein (PF10368; HMM-score: 116.6)
    and 19 more
    Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 22)
    TPR (CL0020) COG6_N; Conserved oligomeric complex COG6, N-terminal (PF06419; HMM-score: 16.7)
    no clan defined Med4; Vitamin-D-receptor interacting Mediator subunit 4 (PF10018; HMM-score: 14.1)
    APG6_N; Apg6 coiled-coil region (PF17675; HMM-score: 13.7)
    DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 12.4)
    BORCS7; BLOC-1-related complex sub-unit 7 (PF16088; HMM-score: 12.1)
    DmpA_ArgJ (CL0635) MoCoBD_2; Molybdopterin cofactor-binding domain (PF20256; HMM-score: 12)
    TerB (CL0414) TerB; Tellurite resistance protein TerB (PF05099; HMM-score: 11.2)
    no clan defined BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 11.1)
    DASH_Duo1; DASH complex subunit Duo1 (PF08651; HMM-score: 10.6)
    IL31; Interleukin 31 (PF15209; HMM-score: 10.4)
    ABC_tran_CTD; ABC transporter C-terminal domain (PF16326; HMM-score: 9.9)
    Golgi-transport (CL0145) BAR_3; BAR domain of APPL family (PF16746; HMM-score: 8.7)
    MazG (CL0231) Nuf2_DHR10-like; Nuf2, DHR10-like domain (PF18595; HMM-score: 8.4)
    no clan defined Spc7; Spc7 kinetochore protein (PF08317; HMM-score: 7.6)
    P-loop_NTPase (CL0023) AAA_13; AAA domain (PF13166; HMM-score: 7.2)
    no clan defined UPF0242; Uncharacterised protein family (UPF0242) N-terminus (PF06785; HMM-score: 7.1)
    Cyclin (CL0065) DUF3452; Domain of unknown function (DUF3452) (PF11934; HMM-score: 6.8)
    no clan defined V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 5.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.8972
    • Cell wall & surface Score: 0.0592
    • Extracellular Score: 0.0435
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: VLLAGCT
  • SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.000447
    • TAT(Tat/SPI): 0.000065
    • LIPO(Sec/SPII): 0.999242
    • Cleavage Site: CS pos: 19-20. LAG-CT. Pr: 0.9999
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPIKTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSEQDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLNQNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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