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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01361
  • pan locus tag?: SAUPAN003751000
  • symbol: SAOUHSC_01361
  • pan gene symbol?: msrR
  • synonym:
  • product: transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01361
  • symbol: SAOUHSC_01361
  • product: transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 1304855..1305838
  • length: 984
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGATAAAGAAACTAATGACAACGAATATAGACGTCAAAGTGAACATCGCACTTCGGCG
    CCTAAGCGAAAAAAGAAGAAGAAAATTAGGAAATTACCTATCATTCTTCTGATTGTTGTA
    ATTTTACTTATCGCATTAGTTGTATATATTGTACATAGTTACAATAGCGGTGTAGAATAT
    GCCAAGAAACATGCGAAAGATGTTAAAGTACATCAATTTAATGGACCAGTAAAAAATGAT
    GGTAAAATTTCTATTCTTGTACTCGGTGCAGATAAAGCACAAGGTGGACAATCAAGAACA
    GATTCTATCATGGTTGTTCAATATGACTTTATCAATAAAAAGATGAAAATGATGTCTGTC
    ATGCGTGATATTTATGCAGATATTCCAGGATATGGAAAACACAAAATTAATTCAGCATAC
    GCTTTAGGTGGTCCAGAGCTACTTAGAAAAACACTTGATAAAAATTTAGGAATTAATCCT
    GAATATTATGCAGTAGTTGATTTTACTGGATTTGAGAAAATGATTGATGAATTAATGCCT
    GAAGGTGTACCAATTAATGTCGAAAAAGATATGTCGAAAAATATTGGTGTATCTTTGAAA
    AAGGGTAACCATAGGTTGAATGGTAAAGAATTACTTGGTTATGCAAGATTCCGTCACGAC
    CCTGAAGGTGACTTCGGACGCGTGCGACGTCAGCAACAAGTGATGCAAACATTGAAAAAA
    GAAATGGTTAATTTTAGAACAGTTGTTAAATTACCAAAAGTTGCAGGTATTTTAAGAGGC
    TATGTGAATACAAACATTCCTGATTCAGGGATTTTCCAAACAGGTTTGAGTTTTGGTATC
    CGAGGTGAAAAAGATGTTAAGTCATTGACTGTGCCAATCAAGAACTCATACGAAGATGTC
    AATACAAATACTGATGGTAGTGCATTACAGATTAATAAAAACACAAATAAACAAGCTATT
    AAAGACTTTTTAGATGAAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01361
  • symbol: SAOUHSC_01361
  • description: transcriptional regulator
  • length: 327
  • theoretical pI: 10.2483
  • theoretical MW: 36970.5
  • GRAVY: -0.514679

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions Other cell envelope-related function transcriptional attenuator common domain (TIGR00350; HMM-score: 117.4)
    and 3 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssA (TIGR03927; HMM-score: 15)
    Cellular processes Cellular processes Pathogenesis type II secretion system protein G (TIGR01710; HMM-score: 8.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein G (TIGR01710; HMM-score: 8.2)
  • TheSEED  :
    • Peptide methionine sulfoxide reductase regulator MsrR
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Peptide methionine sulfoxide reductase regulator MsrR
  • PFAM:
    no clan defined LytR_cpsA_psr; Cell envelope-related transcriptional attenuator domain (PF03816; HMM-score: 170.4)
    and 7 more
    DUF929; Domain of unknown function (DUF929) (PF06053; HMM-score: 16)
    DUF3671; Protein of unknown function (PF12420; HMM-score: 15.4)
    DUF5383; Family of unknown function (DUF5383) (PF17355; HMM-score: 15)
    Zn_Beta_Ribbon (CL0167) DUF2116; Uncharacterized protein containing a Zn-ribbon (DUF2116) (PF09889; HMM-score: 14)
    no clan defined DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 12.7)
    Orf78; Orf78 (ac78) (PF06024; HMM-score: 12.6)
    Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 9.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.057495
    • TAT(Tat/SPI): 0.000446
    • LIPO(Sec/SPII): 0.002712
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLIALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDSIMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINPEYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFRHDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGLSFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Jutta Rossi, Markus Bischoff, Akihito Wada, Brigitte Berger-Bächi
MsrR, a putative cell envelope-associated element involved in Staphylococcus aureus sarA attenuation.
Antimicrob Agents Chemother: 2003, 47(8);2558-64
[PubMed:12878519] [WorldCat.org] [DOI] (P p)

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