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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02799
  • pan locus tag?: SAUPAN006109000
  • symbol: SAOUHSC_02799
  • pan gene symbol?: sarT
  • synonym:
  • product: accessory regulator T

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02799
  • symbol: SAOUHSC_02799
  • product: accessory regulator T
  • replicon: chromosome
  • strand: -
  • coordinates: 2575197..2575517
  • length: 321
  • essential: no [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    ATGAAAAGAGTATTAACTACATATGAGCTGCGTAAATATTTAAAAAAATATTTTTGTCTT
    ACATTAGATAATTATTTAGTATTAGCATATTTAGATGTATTTAAAAATGATGAAGGTAAA
    TATTTTATGAGAGACATTATAAGTTATATCGGTATAGACCAATCTAGAATTGTTAAAAGC
    GTAAAAGATTTATCAAAAAAGGGTTACTTGAATAAATGTAGAGACCCACATGATAGTAGA
    AATGTAATCATTGTTGTTTCTGTAAAACAACACAATTATATTAAAAATCTTTTGAGTGAA
    ATAAATATTAATGAAACATAA
    60
    120
    180
    240
    300
    321

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02799
  • symbol: SAOUHSC_02799
  • description: accessory regulator T
  • length: 106
  • theoretical pI: 9.79423
  • theoretical MW: 12615.7
  • GRAVY: -0.345283

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 114.9)
    and 1 more
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 12.7)
  • TheSEED  :
    • Transcriptional regulator SarT (Staphylococcal accessory regulator T)
  • PFAM:
    HTH (CL0123) HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 28.4)
    MarR_2; MarR family (PF12802; HMM-score: 26.9)
    and 4 more
    MarR; MarR family (PF01047; HMM-score: 19.6)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 18.7)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 13.3)
    B-block_TFIIIC; B-block binding subunit of TFIIIC (PF04182; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003444
    • TAT(Tat/SPI): 0.000092
    • LIPO(Sec/SPII): 0.000698
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRVLTTYELRKYLKKYFCLTLDNYLVLAYLDVFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQHNYIKNLLSEININET

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

K A Schmidt, A C Manna, S Gill, A L Cheung
SarT, a repressor of alpha-hemolysin in Staphylococcus aureus.
Infect Immun: 2001, 69(8);4749-58
[PubMed:11447147] [WorldCat.org] [DOI] (P p)Adhar C Manna, Ambrose L Cheung
sarU, a sarA homolog, is repressed by SarT and regulates virulence genes in Staphylococcus aureus.
Infect Immun: 2003, 71(1);343-53
[PubMed:12496184] [WorldCat.org] [DOI] (P p)Katherine A Schmidt, Adhar C Manna, Ambrose L Cheung
SarT influences sarS expression in Staphylococcus aureus.
Infect Immun: 2003, 71(9);5139-48
[PubMed:12933857] [WorldCat.org] [DOI] (P p)

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