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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02809
  • pan locus tag?: SAUPAN006132000
  • symbol: SAOUHSC_02809
  • pan gene symbol?: gntR
  • synonym:
  • product: gluconate operon transcriptional repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02809
  • symbol: SAOUHSC_02809
  • product: gluconate operon transcriptional repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 2587959..2588639
  • length: 681
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATGTATGGATATCCAGAGAAATGGTTGGAAGGTATGACAACTGGAGAAGGTATCGCG
    GCAGAATTACGCTTAGGCATTGTGAATGGTCACATAGCTGAAGGTACGTTACTCACTGAA
    AATCAAATGGCAAAGCAATTTAATGTGAGTCGTTCGCCAATTCGAGATGCATTTAAATTA
    TTGCAACAAAATCAACTCATCCAATTAGAAAGAATGGGTGCACATGTGTTGCCGTTTGGG
    GAACAAGAAAAGAAAGAAATGTATGATTTGCGACTGATGTTAGAGTCATTTGCATTTTCA
    AGAGTTAAAAATCAAGAGCGACTACCTATCGTAAAAGAAATGAAGAAACAACTTGAAATG
    ATGAAAGTGGCAGTAAAATTTGAGGATGCAGAATCATTTACGAAGCATGACTTTGAATTT
    CATGAAACATTAATCAAAGCATCTAATCATCAATATTTAAACTCATTTTGGAGTCATTTA
    AAACCAGTAATGATGGCACTCGTTTTAACATCCATGCGACAACGTATGCAACAGAACCCG
    CAAGATTTTGAACGCATACATCATAATCATCAAGTGTTTATTGATGCAGTGGAACAATAC
    GACAGTCAAATTTTGAAAGAAGCGTTTCATTTAAATTTCGACGATGTTGGTAAAGATATT
    GAAGGATTTTGGTTGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    681

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02809
  • symbol: SAOUHSC_02809
  • description: gluconate operon transcriptional repressor
  • length: 226
  • theoretical pI: 6.35132
  • theoretical MW: 26595.4
  • GRAVY: -0.469912

⊟Function[edit | edit source]

  • TIGRFAM:
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 85.1)
    and 8 more
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 26.5)
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 16.2)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 16.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
    Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
  • TheSEED  :
    • Gluconate operon transcriptional repressor
    Carbohydrates Monosaccharides D-gluconate and ketogluconates metabolism  Gluconate operon transcriptional repressor
  • PFAM:
    FadR-C (CL0388) FCD; FCD domain (PF07729; HMM-score: 71.6)
    and 5 more
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 47.3)
    HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 19.7)
    FadR-C (CL0388) FadR_C; FadR C-terminal domain (PF07840; HMM-score: 16.2)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 13.3)
    ATP-grasp (CL0179) DUF1297; Domain of unknown function (DUF1297) (PF06973; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Gluconate
  • genes regulated by GntR*, TF important in Gluconate utilizationRegPrecise
    repression
    gntP* < S1093 < gntK* < gntR*
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003314
    • TAT(Tat/SPI): 0.000539
    • LIPO(Sec/SPII): 0.000578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMAKQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRVKNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHLKPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGKDIEGFWLN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA* regulon, GntR* (repression) regulon
    CcpA*(TF)important in Carbon catabolism; RegPrecise 
    GntR*(TF)important in Gluconate utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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