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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2516 [new locus tag: SACOL_RS13175 ]
  • pan locus tag?: SAUPAN006132000
  • symbol: gntR
  • pan gene symbol?: gntR
  • synonym:
  • product: gluconate operon transcriptional repressor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2516 [new locus tag: SACOL_RS13175 ]
  • symbol: gntR
  • product: gluconate operon transcriptional repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 2576016..2576696
  • length: 681
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATGTATGGATATCCAGAGAAATGGTTGGAAGGTATGACAACTGGAGAAGGTATCGCG
    GCAGAATTACGCTTAGGCATTGTGAATGGTCACATAGCTGAAGGTACGTTACTCACTGAA
    AATCAAATGGCAAAGCAATTTAATGTGAGTCGTTCGCCAATTCGAGATGCATTTAAATTA
    TTGCAACAAAATCAACTCATCCAATTAGAAAGAATGGGTGCACATGTGTTGCCGTTTGGG
    GAACAAGAAAAGAAAGAAATGTATGATTTGCGACTGATGTTAGAGTCATTTGCATTTTCA
    AGAGTTAAAAATCAAGAGCGACTACCTATCGTAAAAGAAATGAAGAAACAACTTGAAATG
    ATGAAAGTGGCAGTAAAATTTGAGGATGCAGAATCATTTACGAAGCATGACTTTGAATTT
    CATGAAACATTAATCAAAGCATCTAATCATCAATATTTAAACTCATTTTGGAGTCATTTA
    AAACCAGTAATGATGGCACTCGTTTTAACATCCATGCGACAACGTATGCAACAGAACCCG
    CAAGATTTTGAACGCATACATCATAATCATCAAGTGTTTATTGATGCAGTGGAACAATAC
    GACAGTCAAATTTTGAAAGAAGCGTTTCATTTAAATTTCGACGATGTTGGTAAAGATATT
    GAAGGATTTTGGTTGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    681

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2516 [new locus tag: SACOL_RS13175 ]
  • symbol: GntR
  • description: gluconate operon transcriptional repressor
  • length: 226
  • theoretical pI: 6.35132
  • theoretical MW: 26595.4
  • GRAVY: -0.469912

⊟Function[edit | edit source]

  • TIGRFAM:
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 85.1)
    and 8 more
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 26.5)
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 16.2)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 16.2)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
    Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
  • TheSEED  :
    • Gluconate operon transcriptional repressor GntR, FadR family
    Carbohydrates Monosaccharides D-gluconate and ketogluconates metabolism  Gluconate operon transcriptional repressor
  • PFAM:
    FadR-C (CL0388) FCD; FCD domain (PF07729; HMM-score: 71.6)
    and 5 more
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 47.3)
    HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 19.7)
    FadR-C (CL0388) FadR_C; FadR C-terminal domain (PF07840; HMM-score: 16.2)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 13.3)
    ATP-grasp (CL0179) DUF1297; Domain of unknown function (DUF1297) (PF06973; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Gluconate
  • genes regulated by GntR, TF important in Gluconate utilizationRegPrecise
    repression
    gntP < gntK < gntR
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003314
    • TAT(Tat/SPI): 0.000539
    • LIPO(Sec/SPII): 0.000578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMAKQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRVKNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHLKPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGKDIEGFWLN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, GntR (repression) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 
    GntR(TF)important in Gluconate utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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