PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2403 [new locus tag: NWMN_RS13820 ]
• pan locus tag^?: SAUPAN006132000
• symbol: gntR
• pan gene symbol^?: gntR
• synonym:
• product: gluconate operon transcriptional repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2403 [new locus tag: NWMN_RS13820 ]
• symbol: gntR
• product: gluconate operon transcriptional repressor
• replicon: chromosome
• strand: -
• coordinates: 2645363..2646043
• length: 681
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331970 NCBI
• RefSeq: YP_001333437 NCBI
• BioCyc:
• MicrobesOnline: 3708005 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATGTATGGATATCCAGAGAAATGGTTGGAAGGTATGACAACTGGAGAAGGTATCGCG
  GCAGAATTACGCTTAGGCATTGTGAATGGTCACATAGCTGAAGGTACGTTACTCACTGAA
  AATCAAATGGCAAAGCAATTTAATGTGAGTCGTTCGCCAATTCGAGATGCATTTAAATTA
  TTGCAACAAAATCAACTCATCCAATTAGAAAGAATGGGTGCACATGTGTTGCCGTTTGGG
  GAACAAGAAAAGAAAGAAATGTATGATTTGCGACTGATGTTAGAGTCATTTGCATTTTCA
  AGAGTTAAAAATCAAGAGCGACTACCTATCGTAAAAGAAATGAAGAAACAACTTGAAATG
  ATGAAAGTGGCAGTAAAATTTGAGGATGCAGAATCATTTACGAAGCATGACTTTGAATTT
  CATGAAACATTAATCAAAGCATCTAATCATCAATATTTAAACTCATTTTGGAGTCATTTA
  AAACCAGTAATGATGGCACTCGTTTTAACATCCATGCGACAACGTATGCAACAGAACCCG
  CAAGATTTTGAACGCATACATCATAATCATCAAGTGTTTATTGATGCAGTGGAACAATAC
  GACAGTCAAATTTTGAAAGAAGCGTTTCATTTAAATTTCGACGATGTTGGTAAAGATATT
  GAAGGATTTTGGTTGAATTAA
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⊟Protein

⊟General

• locus tag: NWMN_2403 [new locus tag: NWMN_RS13820 ]
• symbol: GntR
• description: gluconate operon transcriptional repressor
• length: 226
• theoretical pI: 6.35132
• theoretical MW: 26595.4
• GRAVY: -0.469912

⊟Function

• TIGRFAM:
  phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 85.1)
  and 8 more
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 26.5)
  Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
  Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 24.4)
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 16.2)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 16.2)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
  Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
  Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 12.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  FadR-C (CL0388) FCD; FCD domain (PF07729; HMM-score: 71.6)
  and 5 more
  HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 47.3)
  HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 19.7)
  FadR-C (CL0388) FadR_C; FadR C-terminal domain (PF07840; HMM-score: 16.2)
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 13.3)
  ATP-grasp (CL0179) DUF1297; Domain of unknown function (DUF1297) (PF06973; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: Gluconate
• genes regulated by GntR, TF important in Gluconate utilizationRegPrecise

  repression

  gntP < gntK < gntR

  transcription unit transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003314
  • TAT(Tat/SPI): 0.000539
  • LIPO(Sec/SPII): 0.000578
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222615 NCBI
• RefSeq: YP_001333437 NCBI
• UniProt: A0A0H3KAE7 UniProt

⊟Protein sequence

• MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMAKQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRVKNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHLKPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGKDIEGFWLN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: gntP < gntK < gntR < NWMN_2404

⊟Regulation

• regulators: CcpA regulon, GntR (repression) regulon

  CcpA	(TF)	important in Carbon catabolism; RegPrecise
  GntR	(TF)	important in Gluconate utilization; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications