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⊟Summary[edit | edit source]
- pan ID?: SAUPAN001831000
- symbol?: —
- synonym:
- description?: phage major capsid protein
- phage major capsid protein
- phiSLT ORF387-like protein
- prophage L54a, major capsid protein
- bacteriophage capsid protein
- phage major capsid protein, HK97 family
- phage major capsid protein HK97
- phage protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 2276138..2277301
- synteny block?: BlockID0012070
- occurrence?: in 44% of 34 strains
mcp : serogroup A prophage capsid scaffold and major capsid coat fusion protein [1]
• A crystal structure is available : 7RWZ
Staphylococcal prophage capsids are typically assembled from major capsid proteins and scaffold proteins with scaffold proteins being lost during capsid maturation. Serogroup A capsids incorporate both functionalities within the same protein with the δ domain performing the scaffolding activity. 535 MCP monomers are involved in the assembly starting from a nucleus of 5 hexamers, extended into a prolate capsid by cylindrical arrangements of six rings of ten hexamers. After assembly, the δ scaffolding domain is cleaved by a crotonase-like head maturation protease between lysine-128 and leucine-129 generating a mature capsid.
⊟Orthologs[edit | edit source]
04-02981:
—
08BA02176:
C248_0352
11819-97:
MS7_1465
6850:
—
71193:
—
ECT-R 2:
—
ED133:
—
ED98:
SAAV_2029
HO 5096 0412:
SAEMRSA15_13710
JH1:
SaurJH1_1082
JH9:
SaurJH9_1062
JKD6008:
—
JKD6159:
—
LGA251:
—
M013:
—
MRSA252:
SAR1516
MSHR1132:
—
MSSA476:
SAS0935
Mu3:
—
Mu50:
—
MW2:
MW1398
RF122:
—
ST398:
SAPIG0376
T0131:
—
TCH60:
HMPREF0772_11693
TW20:
—
USA300_TCH1516:
USA300HOU_1450
VC40:
—
⊟Genome Viewer[edit | edit source]
| COL | |
| NCTC8325 | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.505)
COL MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLETEKAGLQQRFNIVERQV
NCTC8325 MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLETEKAGLQQRFNIVERQV
USA300_FPR3757 MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLETEKAGLQQRFNIVERQV
************************************************************
COL QDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPNEFEKPSMEAQRLLHALPT
NCTC8325 QDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPNEFEKPSMEAQRLLHALPT
USA300_FPR3757 QDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPNEFEKPSMEAQRLLHALPT
************************************************************
COL GNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGLEIPRVSYTLDDDDFITDVET
NCTC8325 GNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGLEIPRVSYTLDDDDFITDVET
USA300_FPR3757 GNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGLEIPRVSYTLDDDDFITDVET
************************************************************
COL AKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVNWVENALQSGLAAKERKDALAVS
NCTC8325 AKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVNWVENALQSGLAAKERKDALAVS
USA300_FPR3757 AKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVNWVENALQSGLAAKERKDALAVS
************************************************************
COL PKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHEDYRDNATIYMRYADYVKIISVLSN
NCTC8325 PKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHEDYRDNATIYMRYADYVKIISVLSN
USA300_FPR3757 PKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHEDYRDNATIYMRYADYVKIISVLSN
************************************************************
COL GTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNYFGINYDGTTYDTDKDVKKGEYLFVLT
NCTC8325 GTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNYFGINYDGTTYDTDKDVKKGEYLFVLT
USA300_FPR3757 GTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNYFGINYDGTTYDTDKDVKKGEYLFVLT
************************************************************
COL AWYDQQRTLDSAFRIAKAKENTGPLPS
NCTC8325 AWYDQQRTLDSAFRIAKAKENTGPLPS
USA300_FPR3757 AWYDQQRTLDSAFRIAKAKENTGPLPS
***************************
- ↑ R L Duda, J Hempel, H Michel, J Shabanowitz, D Hunt, R W Hendrix
Structural transitions during bacteriophage HK97 head assembly.
J Mol Biol: 1995, 247(4);618-35
[PubMed:7723019] [WorldCat.org] [DOI] (P p)Michael S Spilman, Priyadarshan K Damle, Altaira D Dearborn, Cynthia M Rodenburg, Jenny R Chang, Erin A Wall, Gail E Christie, Terje Dokland
Assembly of bacteriophage 80α capsids in a Staphylococcus aureus expression system.
Virology: 2012, 434(2);242-50
[PubMed:22980502] [WorldCat.org] [DOI] (I p)Altaira D Dearborn, Erin A Wall, James L Kizziah, Laura Klenow, Laura K Parker, Keith A Manning, Michael S Spilman, John M Spear, Gail E Christie, Terje Dokland
Competing scaffolding proteins determine capsid size during mobilization of Staphylococcus aureus pathogenicity islands.
Elife: 2017, 6;
[PubMed:28984245] [WorldCat.org] [DOI] (I e)James L Kizziah, Keith A Manning, Altaira D Dearborn, Erin A Wall, Laura Klenow, Rosanne L L Hill, Michael S Spilman, Scott M Stagg, Gail E Christie, Terje Dokland
Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids.
Viruses: 2017, 9(12);
[PubMed:29258203] [WorldCat.org] [DOI] (I e)N'Toia C Hawkins, James L Kizziah, José R Penadés, Terje Dokland
Shape shifter: redirection of prolate phage capsid assembly by staphylococcal pathogenicity islands.
Nat Commun: 2021, 12(1);6408
[PubMed:34737316] [WorldCat.org] [DOI] (I e)